Proceedings of the 1st International Conference in One Health (ICOH 2017)

The Importance of Oocyte and Sperm Cryopreservation of Indonesian Genetic Resources of Local Sheep and Goat

Authors
Gatot Ciptadi, Muh. Nur Ihsan, Sri Rahayu
Corresponding Author
Gatot Ciptadi
Available Online July 2017.
DOI
https://doi.org/10.2991/icoh-17.2018.4How to use a DOI?
Keywords
Genetic, Frozen Sperm, Oocytes, Small Ruminant, Indonesia.
Abstract
Animal breeding, genetic and reproduction are facing challenges with more powerful and useful methods for creating new breeds, genetic change and improvement. In the near future, the frozen cells of sperms and oocyte of local breed and animals have a potential to trade at a national, regional and international levels. These research goals were to study the oocyte and sperms cryopreservation of a specific breed, superior and potential of small ruminants for the the conservation of animal genetic resources for both research and commercial purposes. The study focused on the local breeds of goats and sheep breeds (PE and Senduro Goats) that have been selected genetically (chromosome and genetic DNA analysis). Sperm was produced and to be cryopreserved and storage in liquid nitrogen (-196 oC). Variables observed are cell freezing and post-thawing quality as well as viability and live-dead (%t). Test fertility studies have been conducted in vitro testing of sperm quality. Meanwhile, oocytes were isolated from ovary obtained from locally slaughtered house, then matured in vitro and be frozen. The freezing oocytes were tested for their quality and fertility in vitro, base on IVF and cleavage rate (%). The result showed that early genetic analysis has been completed and indicates that the Indonesia small ruminants (PE and Senduro) are categorized have a normal genetically base on chromosome analysis ((2N=60, X=acrosentris, Y=metasentris). Post-thawing quality of sperms is considered good quality with > 40 % motility (SNI 2008). From this research showed that both freezing sperm and oocyte method of Indonesia small ruminants is considered as good and potential as an alternative technology for conserving the valuable genetic materials. It was concluded that the cryopreservation of germ cells is an attractive and effective conservation the genetic resources of local, superior animal, rare as well as for endangered animals.
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Proceedings
Part of series
Advances in Health Sciences Research
Publication Date
July 2017
ISBN
978-94-6252-481-1
ISSN
2468-5739
DOI
https://doi.org/10.2991/icoh-17.2018.4How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Gatot Ciptadi
AU  - Muh. Nur Ihsan
AU  - Sri Rahayu
PY  - 2017/07
DA  - 2017/07
TI  - The Importance of Oocyte and Sperm Cryopreservation of Indonesian Genetic Resources of Local Sheep and Goat
PB  - Atlantis Press
SP  - 14
EP  - 16
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.4
DO  - https://doi.org/10.2991/icoh-17.2018.4
ID  - Ciptadi2017/07
ER  -