Proceedings of the 1st International Conference in One Health (ICOH 2017)

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68 articles

Comparative Study: The Role of Stem Cell In Damaged Cell

Fedik Abdul Rantam, Dwikora Novembri Utomo, Heri Suroto
Stem cell is undifferentiated cell that have some properties like self renewal and plasticity. The other properties are can differentiate into some the specific cell. Stem cell is divided into two kinds of cell base on the resource of stem cell are embryonic stem cell and adult stem cell. Base on the...

Development of Medical Devices Based on Protein Marker for Animal and Human Diseases

Aulanni'am Aulanni'am
The concept of medical devices based on immunochromatography, is a combination of chromatography technique (for separation of sample components based on differences of their movement through the sorbent) and the immunochemical reaction that has been implemented in various ways. In this review, we discuss...

Antimicrobial Resistance and the Alternative of Using Probiotics as Growth Promoter

I Wayan Teguh Wibawan
The countries in the Asia-Pacific region, namely, Australia, Bangladesh, China, India, Indonesia, Japan, Malaysia, Myanmar, Philippines, Republic of Korea, Thailand and Viet Nam, have come together on the occasion of the Tokyo Meeting of Health Ministers on Antimicrobial Resistance (AMR) held in Japan;...

The Importance of Oocyte and Sperm Cryopreservation of Indonesian Genetic Resources of Local Sheep and Goat

Gatot Ciptadi, Muh. Nur Ihsan, Sri Rahayu
Animal breeding, genetic and reproduction are facing challenges with more powerful and useful methods for creating new breeds, genetic change and improvement. In the near future, the frozen cells of sperms and oocyte of local breed and animals have a potential to trade at a national, regional and international...

The Precede-Proceed Model As Proposed Model In The One Health Paradigm

Oedojo Soedirham
One Health is "the collaborative effort of multiple disciplines - working locally, nationally, and globally - to attain optimal health for people, animals and the environment". One Health is a new phrase, but the concept extends back to ancient times. The recognition that environmental factors can impact...

Effects Of Goat Milk Peptide On Immuno Histo Chemistry Profile Of Lung Cancer Rattus Norvegicus

Romziah Sidik, Aulanni'am Aulanni'am
Complete feed with high linoleic acid content may produce goat milk containing high linoleic acid too and it has specific protein with molecular weight of 42 kDa. The isolated milk peptide belong to goat milk containing 3.21% Linoleic acid (F2) and 4.80% (F3) can be promoted as candidate peptide with...

Prevalence And Levels Of Soil Transmitted Helminths (Sth) Infection Associated With Gender And Age In Cakung Public Elementary School On District Binuang Serang Banten Area

Titis Cresnaulan Desiyanti, Ambar Hardjanti, Zwasta Pribadi Mahardhika, Putri Rachmawati, Rizki Fauzi Rahman, Yolanda Intan Farellina, Yudi Wahyudi
Underdeveloping countries have a high prevalence of worm infection. Some causes of this condition are poor environmental sanitation, lack of personal hygiene, consuming food contaminated with worm's eggs and low levels of socioeconomic education. More than 1,5 billion people or 24% of world population...

Selfcare Group Efforts To Increase The Confidence Of Lepers In Jeneponto, South Sulawesi

Mr. Syahridha, Mr. Supriadi
Leprosy, one of negledted tropical diseases, is a contagious disease that is so feared by all of people. It is still become one of the problems that faced by the world society because it can lead to a very complex problem. People who are suffering from leprosy may have disabilities that are settled if...

Detection Rifampicin Resistance Tuberculosis Using Xpert MTB/RIF Assay in Saiful Anwar Hospital Malang

Dwi Yuni Nur Hidayati, Yani Jane Sugiri
Tuberculosis is still problem in Indonesia. The increase number tuberculosis patientsand then multidrug resistance tuberculosis the higher it. Xpert MTB /RIF assay ,an automated real time polymerase chain reaction assay, detects the presence Mycobacterium tuberculosis and rifampicin resistance to rifampicin...

Expression Of Human Neutrophil Gelatinase-Associated Lipocalin (Ngal) On Serum And Urine Sample Of Pre Dialysis Kidney Failure Patients

Retty Ratnawati, Tinny Endang Hernowati, Nurina Titisari, Ahmad Fauzi
Biomarkers for the early prediction of human acute kidney injury (AKI) are clinically important. Recently, neutrophil gelatinase-associated lipocalin (NGAL) has found to be a sensitive biomarker for the prediction of human AKI at a very early stage and the development of AKI after surgery. NGAL is an...

Nigella sativa L seed's Extract Modulates Liver Regeneration by Affecting Endogenous Stem Cells in Liver Fibrosis Model of Rat

Safithri Fathiyah, Putra Suhartono Taat, Ferdiansyah Ferdiansyah
Chronic liver disease commonly result in liver fibrosis and eventually liver cirrhosis. It has been demonstrated that patients with liver cirrhosis whom consumed N. sativa seed'sexperienced an improvement such as increasing of serum albumin level followed by diminished lower extremities oedem and ascites....

Expression of IL-1 on Mucosal Cavity's Chronic Inflammation after Provision of Clarias Batrachus Fish Oil

Theresia Indah Budhy, Istiati Istiati, Intan Vallentien Dwi Hariati
Background. The presence of cell or tissue damage always followed byinflammatory reactions. Inflammatory process should not persist because it can interfere even inhibit regeneration. An increased circulation of IL-1, IL-6 and TNF-alpha happens in chronic inflammation. IL-1 is granulocytes chemoattractant...

Effects of Aloe vera Cream On Skin Wound Healing In Sprague Dawley Rats: The Role of CD4+ And CD8+ Lymphocytes

Yos Adi Prakoso, Kurniasih Kurniasih
Wound is defined as a damage of the normal anatomical structure and function of tissues. Aloe verais one of potential herbal on wound healing. The aim of this study is to explore the effect of topical Aloe veraon skin wound healing in Sprague Dawleyrats. Thirty six male Sprague Dawley rats 150-200 grams...

The Anthelminthic Effectivity of Gandarusa Leaves (Justiciagendarussa Burm. F.) Infusion And Kapok Seed Infusion (Ceibapentandra L.) Against Female Ascarissuum In Vitro

Rita Tjokropranoto, Cherry Azaria, Manasye Jutan, Said Muh.Faros Ghalib
Ascariasis is known as "soil transmitted helminths" which persons were infected by ingesting eggs were passed in the feces. The most prevalence of Ascariasis in school children are more than 30%, with resultant impairment in growth retardation, intellectual, and cognitive development. The purpose of...

Vitamin E Changes Erythrocyte and Leukocyte Levels in Male Rats (Mus Musculus) Due to Strenuous Exercise

Sugiharto Sugiharto, Mohammad Arif Ali, Anies Setiowati
Oxidative stress can be induced by strenuous exercise either in human or in animal, it can damages erythrocyte during and post-exercise. Furthermore, maximum exercise also affecting leukocyte level. In another hand that vitamin E as an agent of antioxidant might be potentially can be a protector for...

Rose Bengal Test and Complement Fixation Test To Detect Human Brucellosis In Occupationally Exposed Groups At Cilawu District, Garut

Risqa Novita
Human brucellosis is a neglected zoonoses disease in Indonesia. The data on human brucellosis prevalence is limited. Brucellosis infects human from infected animals by direct contact, drink milk from infected animals and breath the contaminated air. Farmers, dairy workers, veterinarians and people who...

Protein of Sarcoptes Scabiei var.Caprae Inducing Rabbit's Immune Response and Toll-Like Receptor-2 (TLR-2) as Marker

Nunuk Dyah Retno Lastuti, Fedik Abdul Rantam, Pudji Hastutiek, Dony Chrismanto
This research aims are to detect S.scabiei var.caprae antigenic protein which can induce cellular immune response in rabbit as acquired immunity with TLR-2 as marker. This research was performed in several stages i.e soluble protein S.scabiei var.caprae mites extraction; rabbit immunization by inoculating...

Potential of Lactobacillus casei Shirota Strain as Probiotic toward MDA Levels and Histopathology of Aortic Tissues in Rat with High Cholesterol Diet

Kiswanti Surya Utami, Chanif Mahdi, Aulanni'am Aulanni'am
Hypercholesterolemia is a major risk factor of cardiovascular disease. Hypercholesterolemia is a condition that the cholesterol levels in the body exceed the normal limits. The main factor that causes high cholesterol levels in the body is a high-cholesterol diet. The provision of high-cholesterol diet...

Effectivity 0f Both KIO3 And KI Salt Toward Iodium (I2) Level In Urine, Malondialdehyde (MDA) and Histipathology of Thyroid Gland of Goitrogenic Rats

Chanif Mahdi, Risman Heli, Sasangka Prasetyawan
Goitrogenic a substance that can inhibit the taking of iodine by the thyroid gland, so that the concentration of iodine in the thyroid to be low., is characterized by the inflammation in the gland thyroid area caused an excessive of free radicals. An excessive of free radicals in the body cause oxidative...

Cow's Milk Yogurt Modulate Expression of STAT5, BMI-1 and LGR-5 in ileum of Indomethacine-Induced Inflammatory Bowel Disease (IBD) in Rats.

Masdiana Chendrakasih Padaga, Wibi Riawan, Nur Hamni
Inflammatory bowel disease (IBD) is characterized by a chronic recurrent inflammation of the gastrointestinal tract . The environmental factors affecting IBD include diet, the use of antibiotics and nonsteroidal anti-inflammatory drugs (NSAIDs) and stress. All of which can directly or indirectly affect...

Sensitivity test of Escherichia coli againts extract of Tinospora crispa

Lucia Muslimin, Nurul Rezqi Hazrah, Abdul Wahid Jamaluddin
In general, a bacterium such as Escherichia coli produces a kind of toxic protein which can disrupt intestinal wall. Livestock reacts to these toxins by pumping lots of water into the intestine in order to rinse or flush these toxins. As a result, the livestocks have diarrhea as a body response to remove...

The studies of Leaf extract of Ficus lyrata Warb on antimicrobial activities

Dwi wahyuda Wira, Efri Mardawati, Mochammad Djali, Roostita Balia
The extract of leaf Ficus lyrata Warb has potential as antimicrobial agents, because it contains bioactive components such as polyphenols, tannin and triterpenoid that can inhibit the growth of microbials. The maceration extraction method was used in this study using water as solvent agents, to extract...

Profile Histopathology Analysis of Gastric, Duodenum, Ileum, and Colon of Inflammatory Bowel Disease (IBD) Rat Model

Dhita Evi Aryani, Aulanni'am Aulanni'am, Agri Kaltaria Anisa, Wawid Purwatiningsih
Inflammatory bowel disease (IBD) is a disease that is mediated by the immune system that affects the gastrointestinal tract, especially the colon mucosa. This disease will cause diarrhea and mucosal damage. In general, IBD is caused by viruses and bacteria that infect the gastrointestinal tract, but...

Synthesis and Activity Analysis of 3-(10-Bromodecyl)-5-isopropyl-2-methyl-1,4-benzoquinone: In-silico Approach

Novia Eka Setyatama, Siti Mariyah Ulfa, Hideki Okamoto
The 3-(10-bromodecyl)-5-isopropyl-2-methyl-1,4-benzoquinone (1) have been synthesized and structurally characterized by mass spectrometer (LC-MS), FT-IR, 1H-NMR and 13C-NMR spectra. The synthesis was carried out by refluxed Thymoquinone (TQ) with bromoundecanoic acid in the presence of acetonitrile:water...

Bats As A Viral Reservoir: A Short Review Of Their Ecological Characters And Immune System

Desrayni Hanadhita, Aryani Sismin Satyaningtijas, Srihadi Agung Priyono
Bats are reported as natural reservoir host of several viruses, many of which cause severe human disease. Infected bats usually do not show any clinical symptoms and persistently infected. Bats immunity against viruses is an evolutional process of self-defense created by ecological adaptation between...

The Potential Risk of Viral Transmission Among Flying Foxes, Domestic Animals, and Humans in Southern Coast of West Java, Indonesia

Chaerul Basri, Etih Sudarnika, Abdul Zahid, Srihadi Agung Priyono, Retno D. Soejoedono, Eko M. Z. Arifin, Heru Susetya, Bambang Sumiarto, Yupadee Hengjan, Keisuke Iida, Hitoshi Takemae, Eiichi Hondo
Flying foxes have been considered to be involved in the transmission of serious infectious diseases to humans.This study aimed to know the direct and/or indirect contacts of flying foxes that live in the Indonesian natural conservation area, with domestic animals and humans living in the surrounding...

Genetic Characterization of Avian Influenza Virus Isolated from Infected Commercial Duck in Sidenreng Rappang, South Sulawesi in 2016

Ahmad Nadif, Fachriyan H. Pasaribu, Okti Nadia Poetri, Retno D. Soejoedono, Dwi Desmiyeni Putri
Before outbreaks of avian influenza (AI) among commercial laying duck in Central Java in 2012, duck were considered as reservoir of this virus, the outbreaks reveals that duck were also susceptible for AI infection. The outbreaks among laying ducks in 2012 were caused by AIV subtype H5N1 clade 2.3.2,,...

Subtype Identification Of Avian Influenza Virus Isolated from Laying Duck In Sidenreng Rappang, South Sulawesi With Hemagglutination Inhibition Assay

Bagus Nanang Luwito, Ahmad Nadif, Retno D Soejoedono, I Wayan T Wibawan
Avian Influenza virus (AIV) have caused significant economic losses in poultry sector worldwide. Results of genetic mapping showed that circulating AIV in Indonesia were belong to clade 2.1.1, 2.1.2, 2.1.3 and 2.3.2, however the AIV subtype were merely H5N1.Our study aim to identify the hemagglutinin...

The influence of hunting activity on haematological parameters of local dogs in Tabek Panjang, Baso, West Sumatera, Indonesia

Radhiati Kemala Sari, Triva Murtina Lubis
This study aims to determine whether a physical activity such as hunting wild boars has effects on erythrocyte counts, haemoglobin concentration, and haematocrit values of local dogs (Canis familiaris)in Tabek Panjang, Baso Subdistrict, West Sumatera, Indonesia. The study used ten healthy male local...

Assessment of Profile of Antigenicity and Immunogenicity of Eimeria maxima and Eimeria tenella Through Propagative Level and Histopathological Changes in Site Infection for Exploration of Optimal Low Doses of Bivalent Chicken Coccidiosis Live Vaccine

Endang Suprihati, Muchammad Yunus
Live vaccines group was the first to be studied, due to the fact that live parasites reduce further reinfection. The development of vaccine to control coccidiosis caused by E. maxima and E. tenella in chickens is intensifying because of the increasing threat of drug resistance to anticoccidial agents....

One Health Approach in the Understanding of Possible Diseases Transmission by Fruits Bats

Didik Pramono, Supratikno Supratikno, I Nengah Donny Artika, Faisal Tanjung, Ni Luh Putu Ika Mayasari, Etih Sudarnika, Abdul Zahid Ilyas, Chaerul Basri, Srihadi Agungpriyono
About 75% of contagious diseases in the world are classified as zoonoses. One among wild animals suspected to spread the disease is bat. Bats have an important role in the spreading of viral diseases. Fruit bat is among bat species that may potentially transmit diseases to human. The transmission of...

Haematological study of fruit bat, Cynopterus Tithaecheilus

Anisa Rahma, Desrayni Hanadhita, Andhika Yudha P., Danang D. Cahyadi, Supratikno Supratikno, Hera Maheshwari, Aryani Sismin Satyaningtijas, Srihadi Agungpriyono
Haematological profile is important to determine the physiological condition of an individual, and morphology of red blood cells can also be helpful in making a diagnosis of a disease. The size of red blood cells affects the ability of red blood cells to carry oxygen. In some clinical events shape and...

Human Resources Development Strategy In Brucellosis Diseases Monitoring At Sentra Peternakan Rakyat Cinarabogo, Subang

Ferdi Fathurohman, Enceng Sobari, Fika Ayu Safitri
Running business in group, in Indonesian is Sentra Peternakan Rakyat (SPR), which are sustainably running business together is the hope for all the farmers and also the government to meet the needs of national meats. In the past, some of farmers must disband and terminate its business due to wrong strategy...

Genetic Characterization of Viral Nervous Necrosis Infects Tilapia (Oreochromis sp.) in Indonesia

Uun Yanuhar, Novia Christi, Diana Arfiati
Tilapia (Oreochromis sp.) is the most important cultured fish in the 21 century and Indonesia is the country's second largest tilapia producer after China. It make Indonesia should be more concerned with any possible attack diseases that can infect both from the class of parasites, fungi, bacteria, and...

Genetic Variation of Local Ettawah Crossbreed Goats in two different breeding

Mudawamah Mudawamah, I.D. Ratnaningtyas, M.Z. Fadli, Aulanni'am Aulanni'am, G. Ciptadi
Breeding system in goats in Indonesia applying had two models of breeding with owned bucks by breeders or breeding assisted by bucks selected from the government in the form of frozen semen. Problems interesting note was how the genetic variation of the population of the goat. The purpose of this study...

Hypothesis: Relationship of skin morphology and cutaneous scarless wound healing in sunda porcupine (Hystrix javanica)

Andhika Y Prawira, Supratikno Supratikno, Savitri Novelina, Srihadi Agungpriyono
Sunda porcupine (Hystrix javanica) is one among the wild mammals of Indonesia. The porcupine skin shows peculiar appearance by the presence of quills, spine like structure, instead of hair, that commonly found in mammals. On the other hand, our previous preeliminary study observed a relatively quick...

Primer design and in silico analysis of endoglucanase gene for Bacillus genus

Dewi Yuliani, Akyunul Jannah
Endoglucanase is an enzyme initiating hydrolysis of cellulose. The enzyme has been applied in various fields, such as medicine and food. Previous research has been isolated cellulolytic bacteria that are capable to produce endoglucanase, i.e. Bacillus brevis and Bacillus cereus from bekatul. Determination...

Electroencephalogram recording in Garut sheep: effect of the use of xylazine in small ruminants

Dian Vidiastuti, Harry Soehartono, Deni Novianto
Minor procedures which are not painfull but require the animal to be relatively immobile may be performed with the aid of a sedative. In practical terms, sedative agent cause some degree of drowsiness until lost of consciousness. Xylazine used as a sedative and anaesthetic induction by inhibiting the...

Pathotypic and Phylogenetic Analysis of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia

Dwi Desmiyeni Putri, Ekowati Handharyani, Retno Damajanti Soejoedono, Agus Setiyono, Ni Luh Putu Ika Mayasari, Okti Nadia Poetri
Newcastle disease (ND) is a highly contagious disease of poultry worldwide, caused by Newcastle Disease virus (NDV), also kown as Avian Paramyxovirus Type1 (OIE. 2012). Despite vaccination, outbreaks of ND in vaccinated chicken flocks in Indonesia have been regularly reported. The emergence of new virulent...

Teat End Condition and Hyperkeratosis at Different Lactation Periods

Herwin Pisestyani, RP Agus Lelana, Advis Dwi Saputra, Retno Wulansari, Afton Atabany, Mirnawati B. Sudarwanto
As a producer of milk, dairy cows must be in good health condition. This study was aimed to assess the influence of hand milking at difference lactation and udder quarter towards teat end condition and hyperkeratosis on dairy cattle in Kunak Bogor and Pasir Jambu Bandung. Object used in the study are...

The potency of Binahong leaves (Anredera cordifolia (Ten.) Steenis) to recovery process of wound in the livestock

Rita Purwasih, Fika Ayu Safitri
Anredera cordifolia (Ten.) Steenis, in Indonesian is binahong, is a creeping plant with a length can reach 6 meters. Binahong originated from the United Stated and be classified in Family Basellaceae. In Indonesia, binahong often be used for traditional medicines to treat diabetes, relieve shortness...

Antibacterial activity of Muntingia Calabura Lam. against some selected bacteria cousing mastistis

Puguh Sujarwodojo, Thohari Imam, Tri Saputtra Firmansyah, Ridhowi Aswah
The use of herbal plants are widely knows to be used in the treatment of various infectious diseases throughout the history of mankind. The aim of this study was to determine the effectiveness of extract Muntingia Calabura L at different storage (refrigerator vs. room temperature) and time duration (0,2,4,6,8...

Succesfull of Artificial Insemination by Using Chilled Semen on Brahman Cross Cows

Aulia Puspita Anugra Yekti, Enike Dwi Kusumawati, Kuswati Kuswati, Aswah Ridhowi, Herni Sudarwati, Nurul Isnaini, Trinil Susilawati
Successfull of artificial insemination by using frozen semen is relatively low, because of decreasing of sperm motility after frozen process. In this study, the chilled semen was used to increase the succesful of artificial insemination. The purpose of this study was to evaluate the successful of artficial...

The Effect of Sexing Process by Using Density Gradient Centrifugation Percoll and Frozen Method to Sperm Motility and Membrane Damage of Ongole Crossbred Bull

Trinil Susilawati, Enike Dwi Kusumawati, Nurul Isnaini, Aulia Puspita Anugra Yekti, Herni Sudarwati, Aswah Ridhowi
Artificial insemination by using semen sexing aims to improve the genetic quality and to get calf with expected gender. Sexing by using density gradient centrifugation percoll and sedimentation egg whites has been frozen successfully. This study aims to determine the effect of sexing process by using...

Effect of Follicle Size on Metaphase II and Cleavage Rate of Goat Oocyte

Sri Wahjuningsih, Muhammad Nur Ihsan
The aim of this research was to determine the effect of follicle size on Metaphase II and cleavage rate of Goat Oocyte. Ovaries from slaughterhouse transported to the laboratory within 2 h of slaugtering in normal saline. The ovaries were cut and classified two classes of follicle size : small (2-4 mm...

Antifertility Effect of Centella asiatica (L) Urban and Plucea indica (L) Urban on the Number of Follicles, Antioxidant Activity and Hormonal Profile of White Rat's Ovaries

Bayyinatul Muchtaromah, Mukholifah Mukholifah, Ihda Sayidatun Nasiroh, Mujahidin Ahmad, Romaidi Romaidi
Antifertility agents with safety and effectiveness in terms of minimum side effects have always been a subject of debate. Many studies have been conducted on plants to observe the antifertility effect, but majority of them were toxic. Centella asiatica├┐and Plucea indica leaves have been traditionally...

Profiling OVGP Protein in Oviductal Fluid of Kacang Goats in Malang

Herawati Herawati, Aulia Firmawati, Herlina Pratiwi, Nurul Isnaini
Frozen semen suplemented by Oviduct Specific Glycoprotein is a new way to solve the decrease of in vitro fertility on goats caused by polysperm and increase efficiency of artificial insemination on goats. This study was aimed to determine Oviduc Specific Glycoprotein profile in oviductal fluid of Kacang...

Supplementation of Mangosteen (Garcinia mangostana) Pericarp Filtrate in Tris-egg yolk-based Diluent on Buck Sperm Membrane Integrity

Nurul Isnaini, Nuryadi Nuryadi, Eko Nugroho
This study was conducted to evaluate supplementation of Mangosteen (Garcinia mangostana) pericarp filtrate in a tris-egg yolk-based diluent on buck sperm membrane integrity. Semen was collected from 5 bucks by artificial vagina method. Fresh semen evaluated for colour, pH, volume, concentration, mass...

Contamination of Antibiotic Resistant Enterobacteriaceae

Denny Widaya Lukman, Hadri Latif, Herwin Pisestyani, Trioso Purnawarman, Eddy Sukmawinata, Ardilasunu Wicaksono, Chaerul Basri, Etih Sudarnika, Abdul Zahid Ilyas, Mirnawati Sudarwanto
This study was aimed to determine Enterobacteriaceae contamination that were resistant against antibiotics in duck meat which was related with food safety for the consumers. Total of 52 samples of ducks were taken from 5 subdistricts in Bogor District, i.e., Ciomas, Gunung Sindur, Klapanunggal, Jasinga,...

Identification and Characterization of a Cellulase from Bacterial of Indigenous of Rice Bran

Akyunul Jannah, Aulani`am Aulani`am, Tri Ardyati, Suharjono Suharjono
Cellulolytic bacteria have been isolated from rice mill waste (rice bran) that isolates BE 8 and BE 14. Cellulolytic bacteria is a producer of cellulase enzymes involved in the degradation of cellulose waste, textiles, detergents, glucose industrial etc. The purpose of this study was to molecular identification...