Pathotypic and Phylogenetic Analysis of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia
Dwi Desmiyeni Putri, Ekowati Handharyani, Retno Damajanti Soejoedono, Agus Setiyono, Ni Luh Putu Ika Mayasari, Okti Nadia Poetri
Dwi Desmiyeni Putri
Available Online July 2017.
- https://doi.org/10.2991/icoh-17.2018.39How to use a DOI?
- Newcastle disease, pathotypic, phylogenetic analysis, West Java Indonesia
- Newcastle disease (ND) is a highly contagious disease of poultry worldwide, caused by Newcastle Disease virus (NDV), also kown as Avian Paramyxovirus Type1 (OIE. 2012). Despite vaccination, outbreaks of ND in vaccinated chicken flocks in Indonesia have been regularly reported. The emergence of new virulent genotypes implies that distinct genotypes of NDV are simultaneously evolved on different geographic locations across the globe. The genomic diversity of NDV increases the possibility of diagnostic failures, resulting in unidentified infections (Miller et al. 2010). The aim of our study were to characterize pathotype and genotype of NDV isolated in West Java, Indonesia on 2011, 2014, and 2015. Four NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java were used in this study. RT-PCR were used to amplify 254 bp and 1600 bp of the fusion gene (F gene) using three sets of primers. Two sets of primers were spesific to amplify the F protein cleavage site sequence of virulent and avirulent NDV strains. Another set of primer was used to amplify whole genome of F gene and used to sequence. Based on amplification and motif of cleavage site result, two isolates were belong to NDV virulent strains (NDV/Ck/Bogor/011 and NDV/Ck/GnSindur/014) and two isolates were belong to NDV avirulent strains (NDV/Ck/Cianjur/015 and NDV/Ck/Bogor/015). The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R-F117 on NDV virulent strains and 112G-R-Q-G-R-L117 on NDV avirulent strain. Phylogenetic analyses of the F protein revealed that NDV/Ck/Bogor/011 and NDV/Ck/GnSindur/014 belong to genotype VII (sub) genotype (VIIh and VIIi) and NDV/Ck/Cianjur/015 and NDV/Ck/Bogor/015 belong to genotype II. Our study reveal that NDV virulent strain were circulating among vaccinated chicken flocks in West Java, Indonesia. ND outbreaks among vaccinated flocks, suggesting that vaccination strategies have not yet optimal in controlling the virus (Nakamura. Et al. 2014). Result from current study suggested that there is persistence of multiple genotypes of NDV in Indonesia especially genotype VII (Miller et al. 2015) and these findings emphasize the importance of continuous surveillance of NDV in Indonesia. Our result also suggested that virulent NDV strain which belong to genotype VII (sub) genotype (VIIh and VIIi) were circulating among vaccinated flocks in West Java, Indonesia.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Dwi Desmiyeni Putri AU - Ekowati Handharyani AU - Retno Damajanti Soejoedono AU - Agus Setiyono AU - Ni Luh Putu Ika Mayasari AU - Okti Nadia Poetri PY - 2017/07 DA - 2017/07 TI - Pathotypic and Phylogenetic Analysis of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia PB - Atlantis Press SP - 199 EP - 204 SN - 2468-5739 UR - https://doi.org/10.2991/icoh-17.2018.39 DO - https://doi.org/10.2991/icoh-17.2018.39 ID - Putri2017/07 ER -