Identification and Characterization of a Cellulase from Bacterial of Indigenous of Rice Bran

DOI

10.2991/icoh-17.2018.50How to use a DOI?

Keywords

cellulolytic bacteria, rice bran, moleculer identfication and cellulase enzyme.

Abstract

Cellulolytic bacteria have been isolated from rice mill waste (rice bran) that isolates BE 8 and BE 14. Cellulolytic bacteria is a producer of cellulase enzymes involved in the degradation of cellulose waste, textiles, detergents, glucose industrial etc. The purpose of this study was to molecular identification of cellulolytic bacterial isolates from indigenous rice bran using 16S rRNA and characterization of cellulase enzymes produced. The results showed that the BE 8 isolate is Bacillus subtilis with a similarity of 100% of Bacillus subtilis ZJ2. While BE14 isolates is Bacillus cereus with a similarity of 99.9% of Bacillus cereus BPH32, ST307 and JBE0004. Cellulase enzymes produced BE 8 and BE 14 isolate have the highest activity at pH 6 and a temperature of 60 øC. On the influence of metal ions, isolate BE 8 has the highest activity with metal Mg2+, whereas BE 14 isolate has the highest activity with their metal Mn2+.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Akyunul Jannah
AU  - Aulani`am Aulani`am
AU  - Tri Ardyati
AU  - Suharjono Suharjono
PY  - 2017/07
DA  - 2017/07
TI  - Identification and Characterization of a Cellulase from Bacterial of Indigenous of Rice Bran
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 256
EP  - 260
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.50
DO  - 10.2991/icoh-17.2018.50
ID  - Jannah2017/07
ER  -