Proceedings of the 1st International Conference in One Health (ICOH 2017)

Contamination of Antibiotic Resistant Enterobacteriaceae

Authors
Denny Widaya Lukman, Hadri Latif, Herwin Pisestyani, Trioso Purnawarman, Eddy Sukmawinata, Ardilasunu Wicaksono, Chaerul Basri, Etih Sudarnika, Abdul Zahid Ilyas, Mirnawati Sudarwanto
Corresponding Author
Denny Widaya Lukman
Available Online July 2017.
DOI
https://doi.org/10.2991/icoh-17.2018.49How to use a DOI?
Keywords
duck meat, Enterobacteriaceae, total counts, antibiotic resistance
Abstract
This study was aimed to determine Enterobacteriaceae contamination that were resistant against antibiotics in duck meat which was related with food safety for the consumers. Total of 52 samples of ducks were taken from 5 subdistricts in Bogor District, i.e., Ciomas, Gunung Sindur, Klapanunggal, Jasinga, and Parung Panjang. This sample size was calculated based on the assumption of 95% confidence level, 50% predicted prevalence, and 10% standard error. The ducks were collected from small-scale farmers who supplied duck meat to markets, so the ducks were slaughtered by them. The samples of duck meat were handled aseptically and transported in cool box to the laboratory. The total of Enterobacteriaceae was examined using plate count method with violet red bile glucose agar according to Kornacki and Johnson (2001). The examination of antibiotic resistance was done using disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) Guidelines (CLSI 2012). The data was analysed descriptively. The results showed that the total of Enterobacteriacea were 79679 ñ 260463 cfu/g. The majority of Enterobacteriaceae isolated from duck meat (84.6%) showed resistant against 5 of antibiotic (gentamycin, kanamycin, tetracycline, trimethoprim, and ampicillin). The Enterobacteriaceae had resistance against tetracycline (93.2%), trimethoprim (88.6%), and ampicillin (81.8%). Nevertheless, this family was still sensitive againts kanamycin (34.1%) and gentamycin (15.9%). The isolated bacteria (69.2%) showed multi-antibiotic resistant (>3 antibiotics). The antibiotic resistant bacteria could be a potential impact on public health and animal health.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
1st International Conference in One Health (ICOH 2017)
Part of series
Advances in Health Sciences Research
Publication Date
July 2017
ISBN
978-94-6252-481-1
ISSN
2468-5739
DOI
https://doi.org/10.2991/icoh-17.2018.49How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Denny Widaya Lukman
AU  - Hadri Latif
AU  - Herwin Pisestyani
AU  - Trioso Purnawarman
AU  - Eddy Sukmawinata
AU  - Ardilasunu Wicaksono
AU  - Chaerul Basri
AU  - Etih Sudarnika
AU  - Abdul Zahid Ilyas
AU  - Mirnawati Sudarwanto
PY  - 2017/07
DA  - 2017/07
TI  - Contamination of Antibiotic Resistant Enterobacteriaceae
BT  - 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.49
DO  - https://doi.org/10.2991/icoh-17.2018.49
ID  - Lukman2017/07
ER  -