Supplementation of Mangosteen (Garcinia mangostana) Pericarp Filtrate in Tris-egg yolk-based Diluent on Buck Sperm Membrane Integrity
Nurul Isnaini, Nuryadi Nuryadi, Eko Nugroho
Available Online July 2017.
- https://doi.org/10.2991/icoh-17.2018.48How to use a DOI?
- AI, Antioxidant, Dilution, Mangosteen, Semen quality
- This study was conducted to evaluate supplementation of Mangosteen (Garcinia mangostana) pericarp filtrate in a tris-egg yolk-based diluent on buck sperm membrane integrity. Semen was collected from 5 bucks by artificial vagina method. Fresh semen evaluated for colour, pH, volume, concentration, mass motility, motility, viability, sperm abnormality and sperm membrane integrity. Semen was diluted with tris-egg yolk-based extender supplemented with different levels Mangosteen (Garcinia mangostana) pericarp filtrate (0, 10, 20 and 30 %) v/v with the ratio of 1 semen : 9 diluter. Semen used mass motility of 2+ and motility of 70%. Immediately after dilution semen was stored for 4øC and sperm membrane integrity percentage were observed at 0, 24, 48 h and 72 h. The obtained data were analyze with Analysis of Variant (ANOVA) and Least Significant Difference was determined. The experiment was designed using completely random design ( 4 treatments and 10 replications). The results showed that the level of mangosteen pericarp filtrate had very significant effect (P<0,01) on sperm membrane integrity percentage in 0, 24, 48 and 72 h of cooling. It concluded that the best mangsteen pericarp filtrate level for resulting optimal sperm membrane integrity was 10%.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Nurul Isnaini AU - Nuryadi Nuryadi AU - Eko Nugroho PY - 2017/07 DA - 2017/07 TI - Supplementation of Mangosteen (Garcinia mangostana) Pericarp Filtrate in Tris-egg yolk-based Diluent on Buck Sperm Membrane Integrity PB - Atlantis Press SP - 246 EP - 250 SN - 2468-5739 UR - https://doi.org/10.2991/icoh-17.2018.48 DO - https://doi.org/10.2991/icoh-17.2018.48 ID - Isnaini2017/07 ER -