Effects Of Goat Milk Peptide On Immuno Histo Chemistry Profile Of Lung Cancer Rattus Norvegicus

DOI

10.2991/icoh-17.2018.6How to use a DOI?

Keywords

goat milk peptide, linoleic acid, lung cancer, immunohistochemistry, rat.

Abstract

Complete feed with high linoleic acid content may produce goat milk containing high linoleic acid too and it has specific protein with molecular weight of 42 kDa. The isolated milk peptide belong to goat milk containing 3.21% Linoleic acid (F2) and 4.80% (F3) can be promoted as candidate peptide with anti-carcinogenic effect. Twelfth male rats strain Rattusnorvegicusused as the experimental animals with average age about two months old, and body weight around 160 to 200g. The animals were divided into four groups: F0 a healthy rat as a control group. Group F1 rat with artificial lung cancer without goat milk peptide treatment, group F2 rats with artificial lung cancer treated by goat milk peptide (Pep-2) orally. Group F3 rats with artificial lung cancer treated by goat milk peptide (Pep-3) orally. Rattus novergicus in group F1, F2, and F3 were induced by Benzapirine, and incubated for 4 weeks. It was continued to treated with and or without milk peptide as well as the treatment group for one week period. The research was tailored into Complete Randomized Design. The immunohistochemistry expression be scored into apoptosis cell marker perform. Data was analyzed by SPSS software version 13.00. Results of the research showed a significant (P<0.05) good recovery of their lung cancer of rat tissues being to normally in group F2 and F3. By immunohistochemistry technique using antibody Apoptag and DAB substrate, it were performed that alveoli of bronchi septum be compactly, no infiltration of inflammation cell any more, again to form the long bronchi connective tissue and apoptotic cell well performed. However, by continuing treatment by goat milk peptide orally, it be expected an increasing of apoptosis expression rate.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Romziah Sidik
AU  - Aulanni'am Aulanni'am
PY  - 2017/07
DA  - 2017/07
TI  - Effects Of Goat Milk Peptide On Immuno Histo Chemistry Profile Of Lung Cancer Rattus Norvegicus
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 23
EP  - 26
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.6
DO  - 10.2991/icoh-17.2018.6
ID  - Sidik2017/07
ER  -