Comparison Between Oviduct Fluid Protein And Oviduct Epithelia Cell Supplementation In Increasing Oocytes Maturation Rate In Sheep

DOI

10.2991/icoh-17.2018.64How to use a DOI?

Keywords

Oviductal Fluid Protein, Oviductal Ephiteli Cell, in vitro maturation, goat

Abstract

This study aims to increase fertilization rate through improved oocyte quality resulting from in vitro maturation results goats by means of Oviduct Fluid Protein (OFP) and Oviductal Ephitelia Cell (OEP) supplementation. Method used in this study consisted of two phases, 1) OFP collection from oviductal fluid and OEP from shredded oviductal tissue; 2). Oocyte maturation preparation and oocyte observations. It was found that supplementation of oviduct fluid in a concentration of 10% produced maturation rate through the observation of germinal vesicle breakdown (GV), germinal vesicle breakdown GVBD, metaphase 1 (M1), and metaphase 2 (M2) that were significant compared to oviductal ephitelia cell supplementation in concentration of 10% in a maturation medium TCM 199.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Desi Wulansari
AU  - Viski Fitri Hendrawan
AU  - Aulia Firmawati
AU  - Yudit Oktanella
PY  - 2017/07
DA  - 2017/07
TI  - Comparison Between Oviduct Fluid Protein And Oviduct Epithelia Cell Supplementation In Increasing Oocytes Maturation Rate In Sheep
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 317
EP  - 321
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.64
DO  - 10.2991/icoh-17.2018.64
ID  - Wulansari2017/07
ER  -