Nigella sativa L seed's Extract Modulates Liver Regeneration by Affecting Endogenous Stem Cells in Liver Fibrosis Model of Rat

DOI

10.2991/icoh-17.2018.11How to use a DOI?

Keywords

Nigella sativa, Liver fibrosis, CD90 expression, HNF-4 expression, Albumin serum level

Abstract

Chronic liver disease commonly result in liver fibrosis and eventually liver cirrhosis. It has been demonstrated that patients with liver cirrhosis whom consumed N. sativa seed'sexperienced an improvement such as increasing of serum albumin level followed by diminished lower extremities oedem and ascites. The increasing of serum albumin level probably caused by repairment of liver parenchym and or function. Our research aim was evaluation of N. sativa seed's extracts as hepato regenerative agent againts hepatocellular damage induced by hepatotoxins (CCl4). 18 Rats were divided into three groups; normal, liver fibrotic control and liver fibrotic treated with N. sativa groups, 6 rats in each. Liver fibrosis induced by CCl4 intraperitoneal injection @ 1mL/kgBB for 8 weeks, three times in week. After CCl4 induction, administrated ethanol extract of N. sativa seeds by daily intragastric gayage for 4 weeks. At the end of the study, the animal sacrificed, blood and tissue sample were obtained. The level of albumin serum was examinated by spectrophotometry, the mesenchymal stem cell marker CD90, and hepatoblast marker HNF-4 expressions were examinated with immunohistochemistry. It was found that N. sativa's extracts significantly revised on albumin serum level and improved the liver tissue through the increasing of CD90 and HNF-4 expression. From this study, it concluded that Nigella sativa seed's extract exhibited a promising hepatoregenerative effect in the management of liver fibrosis.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Safithri Fathiyah
AU  - Putra Suhartono Taat
AU  - Ferdiansyah Ferdiansyah
PY  - 2017/07
DA  - 2017/07
TI  - Nigella sativa L seed's Extract Modulates Liver Regeneration by Affecting Endogenous Stem Cells in Liver Fibrosis Model of Rat
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 46
EP  - 51
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.11
DO  - 10.2991/icoh-17.2018.11
ID  - Fathiyah2017/07
ER  -