The Effect of Sexing Process by Using Density Gradient Centrifugation Percoll and Frozen Method to Sperm Motility and Membrane Damage of Ongole Crossbred Bull

DOI

10.2991/icoh-17.2018.44How to use a DOI?

Keywords

Artificial insemination, Before freezing motility, Post thawing motility, Ongole crossbred cattle, Sexing sperm.

Abstract

Artificial insemination by using semen sexing aims to improve the genetic quality and to get calf with expected gender. Sexing by using density gradient centrifugation percoll and sedimentation egg whites has been frozen successfully. This study aims to determine the effect of sexing process by using density gradient centrifugation percoll to membrane damage spermatozoa. The materials used were Ongole Crossbred Bull semen with the motility more than 65%. The treatments were; T0 = without sexing; T1 = Sexing. Each treatment was repeated as much as 10 times. The experimental design used was Block Design and descriptive analytics. Media diluent used was andromed@. The percentage of motility was observed by Light microscope magnification 100X, while the damage of sperm was observed by Electrone Scanning microscope (SEM) with a magnification of 10,000 X. The result showed that the percentage of motility were; T0 = 64, 25 ñ 3, 94%; while T1 after sexing on X-sperm and Y- sperm respectively = 53ñ 7,93 % dan 48,55 ñ 8,28%,. The motility before freezing were T0 = 59, 75 ñ 4, 48%, while T1 before Freezing on X-sperm and Y- sperm respectively = 47 ñ 6, 13% dan 42,1 ñ 7,36%. Then post thawing motility on X-sperm and Y- sperm were T0 = 44,2ñ 2,79%, T1 = 31,45ñ 7,20% and 27,45ñ 8,69%. The morphologist of sperm showed that for the raw semen showed the membrane intact spermatozoa while on Y sperm after sexing showed the damaged membranes of the head and in spite of the sperm head to the tail, whereas on the X sperm membrane was not showed the severe damaged. It can be conluded that after freezing both treatments showed the damaged membrane and head with tail no intact on sperm.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Trinil Susilawati
AU  - Enike Dwi Kusumawati
AU  - Nurul Isnaini
AU  - Aulia Puspita Anugra Yekti
AU  - Herni Sudarwati
AU  - Aswah Ridhowi
PY  - 2017/07
DA  - 2017/07
TI  - The Effect of Sexing Process by Using Density Gradient Centrifugation Percoll and Frozen Method to Sperm Motility and Membrane Damage of Ongole Crossbred Bull
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 227
EP  - 231
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.44
DO  - 10.2991/icoh-17.2018.44
ID  - Susilawati2017/07
ER  -