Effect of Follicle Size on Metaphase II and Cleavage Rate of Goat Oocyte
Sri Wahjuningsih, Muhammad Nur Ihsan
Available Online July 2017.
- https://doi.org/10.2991/icoh-17.2018.45How to use a DOI?
- Goat Oocytes, In Vitro Maturation, In Vitro Fertilization, Follicle Size
- The aim of this research was to determine the effect of follicle size on Metaphase II and cleavage rate of Goat Oocyte. Ovaries from slaughterhouse transported to the laboratory within 2 h of slaugtering in normal saline. The ovaries were cut and classified two classes of follicle size : small (2-4 mm diameter ) and large (>4-8 mm diameter) follicles. All culture were done in ten replicates at 38.5oC, 5 % CO2, 95 % humidity. Only fully Cumulus Oocyte Complexes (COCs) and homogenous cytoplasm were used. The COCs were matured for 26 h in TCM-199 medium supplemented with FCS 10 % + EBS 10%. Chi square test were used for statistical analysis. Metaphase II rate was determined by microscope in a sample of COCs denuded,fixed and stained with aceto orcein 1 %. In vitro fertilization was performed using frozen semen (2 X 106 spermatozoa/mL in EBSS solution. The percentage of COCs reaching Metaphase II was 86.42 % (261/302) and 74.33 % (220/300) for oocytes originating from large and small diameter follicles,respectively (P<0.05). After in vitro fertilization , the developmental competence of oocytes dependently increased with follicular size. Higher cleavage rate was obtained from large follicles compare to small follicles (P<0.05).
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Sri Wahjuningsih AU - Muhammad Nur Ihsan PY - 2017/07 DA - 2017/07 TI - Effect of Follicle Size on Metaphase II and Cleavage Rate of Goat Oocyte BT - 1st International Conference in One Health (ICOH 2017) PB - Atlantis Press SN - 2468-5739 UR - https://doi.org/10.2991/icoh-17.2018.45 DO - https://doi.org/10.2991/icoh-17.2018.45 ID - Wahjuningsih2017/07 ER -