Sensitivity test of Escherichia coli againts extract of Tinospora crispa

DOI

10.2991/icoh-17.2018.21How to use a DOI?

Keywords

Antibacterial Tinospora crispa branches, Escherichia coli

Abstract

In general, a bacterium such as Escherichia coli produces a kind of toxic protein which can disrupt intestinal wall. Livestock reacts to these toxins by pumping lots of water into the intestine in order to rinse or flush these toxins. As a result, the livestocks have diarrhea as a body response to remove the toxin in the digestive system. In the presence of these problems, breeders take a measure such as using antibiotics freely. Among breeders, antibiotics are often used freely without knowing the indication and the appropriate dose for the treatment of livestock diseases. It is caused due to the antibiotics easily obtained in animal drugstores which leading to bacterium resistance to these antibiotics. Another alternative done by the breeders is by using plants as medicinal herb such as brotowali (Tinospora crispa). The purpose of this research is to examine Escherichia coli bacterium sensitivity against brotowali extract. This research is descriptive with maceration technique as the method of extract production. The study was conducted by in vitro using Tinospora crispa extract with a concentration of 0.5%, 2%, 8%, and 32%. A positive control using amoxicillin and negative control using DMSO 10%. The result of the research shows that Tinospora crispa extract with the concentration of 0.5% and 2.0% are not more sensitive than amoxicillin, whereas the concentration of 8.0% and 32% are more sensitive than amoxicillin which seen from formed inhibition zone. The content found in Tinospora crispa such as alkaloids, flavonoids, and saponins are antibacterial which has a satisfying effect to kill E.coli.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Lucia Muslimin
AU  - Nurul Rezqi Hazrah
AU  - Abdul Wahid Jamaluddin
PY  - 2017/07
DA  - 2017/07
TI  - Sensitivity test of Escherichia coli againts extract of Tinospora crispa
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 105
EP  - 108
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.21
DO  - 10.2991/icoh-17.2018.21
ID  - Muslimin2017/07
ER  -