Proceedings of the 1st International Conference in One Health (ICOH 2017)

Protein of Sarcoptes Scabiei var.Caprae Inducing Rabbit's Immune Response and Toll-Like Receptor-2 (TLR-2) as Marker

Authors
Nunuk Dyah Retno Lastuti, Fedik Abdul Rantam, Pudji Hastutiek, Dony Chrismanto
Corresponding Author
Nunuk Dyah Retno Lastuti
Available Online July 2017.
DOI
https://doi.org/10.2991/icoh-17.2018.17How to use a DOI?
Keywords
acquired immunity, goat, Sarcoptes scabiei var.caprae , TLR-2
Abstract
This research aims are to detect S.scabiei var.caprae antigenic protein which can induce cellular immune response in rabbit as acquired immunity with TLR-2 as marker. This research was performed in several stages i.e soluble protein S.scabiei var.caprae mites extraction; rabbit immunization by inoculating protein antigen S. scabiei var. caprae with dosage of 200æg, repeated five times as booster in two weeks; examination of TLR-2 expression using direct immunofluorescence technique. Cellular immune response is shown by TLR-2 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The antigenic protein of S.scabiei var.caprae contains ligands, which involve in pathogen associated molecular pattern (PAMP) that can induce cellular immune response in rabbit as with TLR- 2 as marker. It shows that TLR-2 is not only involved in innate immunity but also in acquired immunity.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Proceedings
1st International Conference in One Health (ICOH 2017)
Part of series
Advances in Health Sciences Research
Publication Date
July 2017
ISBN
978-94-6252-481-1
ISSN
2468-5739
DOI
https://doi.org/10.2991/icoh-17.2018.17How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Nunuk Dyah Retno Lastuti
AU  - Fedik Abdul Rantam
AU  - Pudji Hastutiek
AU  - Dony Chrismanto
PY  - 2017/07
DA  - 2017/07
TI  - Protein of Sarcoptes Scabiei var.Caprae Inducing Rabbit's Immune Response and Toll-Like Receptor-2 (TLR-2) as Marker
BT  - 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.17
DO  - https://doi.org/10.2991/icoh-17.2018.17
ID  - Lastuti2017/07
ER  -