Protein of Sarcoptes Scabiei var.Caprae Inducing Rabbit's Immune Response and Toll-Like Receptor-2 (TLR-2) as Marker
Nunuk Dyah Retno Lastuti, Fedik Abdul Rantam, Pudji Hastutiek, Dony Chrismanto
Nunuk Dyah Retno Lastuti
Available Online July 2017.
- https://doi.org/10.2991/icoh-17.2018.17How to use a DOI?
- acquired immunity, goat, Sarcoptes scabiei var.caprae , TLR-2
- This research aims are to detect S.scabiei var.caprae antigenic protein which can induce cellular immune response in rabbit as acquired immunity with TLR-2 as marker. This research was performed in several stages i.e soluble protein S.scabiei var.caprae mites extraction; rabbit immunization by inoculating protein antigen S. scabiei var. caprae with dosage of 200æg, repeated five times as booster in two weeks; examination of TLR-2 expression using direct immunofluorescence technique. Cellular immune response is shown by TLR-2 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The antigenic protein of S.scabiei var.caprae contains ligands, which involve in pathogen associated molecular pattern (PAMP) that can induce cellular immune response in rabbit as with TLR- 2 as marker. It shows that TLR-2 is not only involved in innate immunity but also in acquired immunity.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Nunuk Dyah Retno Lastuti AU - Fedik Abdul Rantam AU - Pudji Hastutiek AU - Dony Chrismanto PY - 2017/07 DA - 2017/07 TI - Protein of Sarcoptes Scabiei var.Caprae Inducing Rabbit's Immune Response and Toll-Like Receptor-2 (TLR-2) as Marker BT - Proceedings of the 1st International Conference in One Health (ICOH 2017) PB - Atlantis Press SP - 79 EP - 83 SN - 2468-5739 UR - https://doi.org/10.2991/icoh-17.2018.17 DO - https://doi.org/10.2991/icoh-17.2018.17 ID - Lastuti2017/07 ER -