Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Anticancer Effect of Red Fruit Fractions Toward Breast Cancer in T47D Cell and Oral Squamous Cancer in KB Cell

Authors
Hana Ratnawati1, *, Yoki Chandra1, Endry Kho1
1Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
*Corresponding author. Email: hana.ratnawati@med.maranatha.edu
Corresponding Author
Hana Ratnawati
Available Online 22 December 2022.
DOI
10.2991/978-94-6463-062-6_33How to use a DOI?
Keywords
Breast cancer; Oral squamous cell cancer; Red fruit fractions; T47D cell; KB cell
Abstract

Indonesia is one of the mega-biodiversity countries, with many plant species that are useful as medicine. Red Fruit (Pandanus conoideus Lam), found in Jayawijaya, Papua, is empirically believed to have numerous health benefits due to having a huge amount of bioactive compounds, especially carotenoids. This study aimed to examine the cytotoxicity effect of red fruit fractions against breast cancer in T47D cells and oral cancer in KB cells compare to doxorubicin. Methanol extracts were fractionated by vacuum liquid chromatography using silica gel H-60 ​​and eluting solvents n-hexane, ethyl-acetate, and methanol in various ratios. In this experimental in-vitro study design, T47D cells and KB cells were induced by four red fruit fractions (I, II, IV, VI) with 6 concentrations (0.0625 μl, 0.125 μl, 0.25 μl, 0.50 μl, 1.00 μl, 2.00 μl) and Doxorubicin as positive control used in this cytotoxicity assay. All data were analyzed by One way ANOVA and Tukey HSD with α = 0.05. The result showed that all red fruit fractions reduced the viability of T47D cells and KB cells in a concentration-dependent manner. Red fruit fraction IV 1 μl had the best cytotoxic effect, which caused 71.48% cell death in T47D cells and 81.03% in KB cells. It can be concluded that all red fruit fractions showed cytotoxicity effect towards breast cancer in T47D cell and oral squamous cell cancer in KB cell. Fraction IV red fruit showed the best effectiveness as anticancer in breast and oral squamous cancer.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 December 2022
ISBN
10.2991/978-94-6463-062-6_33
ISSN
2468-5747
DOI
10.2991/978-94-6463-062-6_33How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Hana Ratnawati
AU  - Yoki Chandra
AU  - Endry Kho
PY  - 2022
DA  - 2022/12/22
TI  - Anticancer Effect of Red Fruit Fractions Toward Breast Cancer in T47D Cell and Oral Squamous Cancer in KB Cell
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 330
EP  - 340
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_33
DO  - 10.2991/978-94-6463-062-6_33
ID  - Ratnawati2022
ER  -