Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Bambang Sugiharto, Asmoro Lelono, Muhammad Akbar Bahar, Syubanul Wathon, Asep Ginanjar Arip, Kartika Senjarini, Ramdhan Putrasetya, Beny Andika, Nadhea Ayu Sukma
All of the articles in this proceedings volume have been presented at the International Conference on Life Science and Biotechnology (ICOLIB) on November 15–16, 2021 at Jember University Indonesia. These articles have been peer-reviewed by the members of the Scientific Committee of ICOLIB and approved...
Analysis of the Impact of 200 µT and 300 µT Extremely Low Frequency (ELF) Magnetic Fields on the Growth Rate of Edamame Plants
Sudarti, Trapsilo Prihandono, Winaning Nur Prihatin, Ilme Mufida Suyono Putri
The Extremely Low Frequency (ELF) magnetic field has an ability to penetrate almost all materials and is non-ionizing radiation so that it can trigger cell proliferation. This study aims to examine the effect of exposure to the 200–300 µT ELF magnetic fields on the growth rate of Edamame plants. This...
Application of Gibberellic Acid (GA3) and Coconut Water with Stratification on Morphological, Anatomical, and Germination of Cherry Seed (Prunus jamasakura)
Ika Fitri Ariyani, Solichatun, Suratman, Sugiyarto
Prunus jamasakura Siebold ex Koidz. (hill cherry) is included in the family of Rosaceae and used for ornamental plants. The dormant cherry seed has become an obstacle since it takes a prolonged time to germinate. Seed germination can be induced by the application of gibberellic acid (GA3) and coconut...
Lipase Production of Aspergillus aculeatus MS. 11 Using Solid State Fermentation on Rubber Seed Press Cake
Maya Nurjanah, Miftahul Ilmi
Lipase is an enzyme that has various use in industrial sector. However, the enzyme production cost is considerably high mainly due to expensive fermentation substrate. Therefore, it is necessary to develop effective media formulation to increase production of lipase enzyme without reducing the amount...
Sex-Embryo Determination Using the Heart Rate as a Non-destructive Method in the Avian Species: Study on Japanese Quail (Cortunix japonica)
Asmoro Lelono, Bambang Sugiharto
Sex determination of the embryo during the incubation phase is crucial in the poultry industry. This study focused on solving one of the crucial problems during the incubation phase in the laying poultry breeding industry, namely determining the sex of the embryos. The early stages of research will focus...
The Habitat Characteristics of Banteng (Bos Javanicus D’alton, 1832) in Pringtali Feeding Ground, Meru Betiri National Park, East Java
Arif Mohammad Siddiq, Hari Sulistiyowati, Tom Reader
Banteng (Bos javanicus) is an endangered native species found in Meru Betiri National Park (MBNP), East Java, Indonesia. This research is aimed to identify the habitat characteristics of banteng at Pringtali feeding ground, Bandealit Resort, MBNP, which is one of the most important habitats for this...
Circan: A Database of Circular RNAs Exploring Chromosomal Linkages in Human Cancers
Gaurav Kumar Bhagat
Circular RNAs (circRNAs) represent a milieu of non-coding RNAs that play a major role in gene regulation, development, and carcinogenesis. Circan is a database of cancer associated circular RNAs. As the interest in circRNA increases, Circan provides a systematic annotation of circRNA dysregulation in...
The Diversity of Indigenous Mushrooms Grow on Decomposed Oil Palm Empty Fruits Bunch at Palm Oil Plantation in Paser Regency, Indonesia
Masitah, Krishna Purnawan Candra, Muhammad Amir Masruhim, Pintaka Kusumaningtyas
The community nearby palm oil plantations have benefited from the indigenous mushroom growing on decomposed oil palm empty fruit bunches (OPEFB). Some mushrooms are edible, but others are known as non-edible due to their toxicity. There is limited scientific information about the biodiversity of indigenous...
The Study of Antibacterial and Antioxidant Activities of Styrax Leaves Fermentation by Aspergillus niger
Sam Muehl Sejahtera Naiborhu, Adelina Manurung, Merry Meryam Martgrita
Styrax leave is a part of Styrax plant that can be used as medicine because it contains potential antibacterial and antioxidant bioactive components. Fermentation has been known increasing antibacterial and antioxidant activity of herbal plants. Aspergillus niger is one of fermentation agents that can...
Optimization of Citric Acid Production by Utilizing Rice Husk Waste as a Substrate Using Submerged Fermentation
Eka Rahmadani Ritonga, Adelina Manurung, Merry Meryam Martgrita
Citric acid is an organic acid and is highly used as a commercial product. The production of citric acid in Indonesia has not met its demand, a cheap and easy method of obtaining raw materials to boost its production is required. Submerged fermentation using Aspergillus niger is a prospective method...
Analysis of Amino Acids, Protein Profile, Calcium and Phosphorus Levels of Upeneus moluccensis Waste (Thorns and Scales)
I Dewa Ayu Ratna Dewanti, I Dewa Ayu Susilawati, Pujiana Endah Lestari, Erawati Wulandari, Ristya Widi Endah Yani, Sunlip Wibisono
Upeneus molucensis is seawater fishes that are consumed by many people so it leaves a lot of waste (including scales and thornss). On the other hand, the utilization of fish waste is still not much. Whereas, waste contains a lot of amino acids, protein, Calcium and Phosphorus. This study analyzed amino...
Identification of Advantages of Indigofera-Pennisetum Intercropping Under Coconut Plantation Based on Dry Matter Yield
Malcky Makanaung Telleng, Wilhelmina Beritan Kaunang, Srimalasinha Sane, Ivonne Maria Untu
The point of this exploration was to decide the benefit of intercropping Indigofera zollingeriana (Iz) and Pennisetum purpureum (Pp) under coconut manor dependent on dry matter yield. This experiment was performed using a completely randomized design (CRD) with six treatment combinations of settlement...
In Vitro Analysis of Human IgG Immune Response Against 31 kDa and 67 kDa Immunogenic Protein from Aedes albopictus Salivary Glands
Syubbanul Wathon, Izza Afkarina, Unzilatir Rohmah, Rike Oktarianti, Kartika Senjarini
Mosquito salivary glands contain protein substances that can facilitate dengue virus transmission. Those proteins have abilities to induce an immune response and specific antibody production. Previous studies showed that 31 kDa and 67 kDa protein fractions from Aedes albopictus salivary glands are immunogenic...
The Apyrase Functional Properties of the 56 kDa Protein from Aedes aegypti Salivary Gland
Rike Oktarianti, Alfan Suhardiansyah, Elisa Erni, Syubbanul Wathon, Kartika Senjarini
Apyrase is an enzyme an inhibit platelet aggregation process, capable of degrading ADP in the process blood feeding and mostly found in hematophagous arthropods. While vector’s blood feeding, this apyrase salivary protein is responsible for inhibiting platelet aggregation in the human host, by hydrolyzing...
The Habitat Suitability of Javan Langur (Trachypithecus auratus E. Geoffroy Saint-Hilaire, 1812) in Kucur Resort at Alas Purwo National Park, Indonesia
Haikal Idris Maulahila, Arif Mohammad Siddiq, Hari Sulistiyowati
The population of Javan Langur is continuous decline as the habitat decreases. One of the remaining protected areas of the Javan Langur is Kucur Resort Alas Purwo National Park (APNP). However, the condition of its habitat is still unclear. This study was conducted to predict the distribution and habitat...
Humoral Immune Response (IgG) of BALB/c Mice (Mus musculus) Post-injection by 56 kDa Immunogenic Protein Extract from the Salivary Glands of Aedes aegypti L.
Aisyah, Rike Oktarianti, Kartika Senjarini, Syubbanul Wathon
The primary mosquito vector of dengue virus is Ae. aegypti, and dengue virus transmitted by saliva mosquito’s during blood feeding. The saliva composed of numerous proteins with capacity to disrupt hemostasis or modulate the host immune response. The previous study identified two immunogenic proteins...
The Effectiveness of Suspension Beta Asarone Mixed with Sillica Nanoparticles in the Mortality of Crocidolomia pavonana
Purwatiningsih, Barlah Rumhayati, Susantin Fajariyah, Raodatul Jannah
The prospect of using botanical insecticides to control the insect pests is very promising. However, there are some factors limiting their efficacy. Short release rate at the point contact, the inherent volatility and vulnerability to oxidation and ultra-violet light are causing phytochemical changes...
Classification of Lymphoma, Benign Lesions, and Carcinoma Using Convolutional Neural Network
Hanina Nuralifa Zahra, Isa Anshori, Hasna Nadila, Hofifa Mulya Utami, Joshua Adi Chandra, Muhammad Rashid Kurniawan, Yunianti Khotimah, Widyawardana Adiprawita, Hermin Aminah Usman, Okky Husain
Lymphoma, carcinoma, and benign lesions are common diseases that have to go through several stages to be detected due to their structural similarity. A common way to distinguish these diseases is by analysing them manually based on the cell images at a certain magnification. However, this method still...
Comparative Study of Convolutional Neural Network Architecture in Lymphoma Detection
Michaella Yosephine, Rafita Erli Adhawiyah, Yasmin Salsabila Kurniawan, Isa Anshori, Ramadhita Umitaibatin, Vegi Faturrahman, Rey Ezra Langelo, Widyawardana Adiprawita, Hermin Aminah Usman, Okky Husain
In this study, we propose an automatic classification of three common types of lymphoma: (1) lymphoma, (2) benign lesion, and (3) carcinoma using lymphoma cell images magnified by 100x and by 400x. A comparative study was performed to find the best architecture to classify lymphoma cell images using...
Deep Learning for Lymphoma Detection on Microscopic Images
Ammar Ammar, Irfan Tito Kurniawan, Resfyanti Nur Azizah, Hafizh Rahmatdianto Yusuf, Antonius Eko Nugroho, Ghani Faliq Mufiddin, Isa Anshori, Widyawardana Adiprawita, Hermin Aminah Usman, Okky Husain
Early lymphoma diagnosis is essential to improve the patients’ survival rate and avoid irreversible damage. Immunohistochemistry-based lymphoma diagnostics is an expensive and time-consuming process, especially in developing countries with limited resources. Image-based lymphoma diagnostics might serve...
Citric Acid Production Optimation from Toba Banana Peel Through Submerged Fermentation by Aspergillus niger Using Central Composite Design
Merry Meryam Martgrita, Adelina Manurung, Herti Novalia Hutapea, Fauziah Balqis Anggi Fitriani
Toba Banana (Musa acuminata Colla) is a local banana in Toba region, North Sumatera, Indonesia. People only utilize its pulp fruit, then its peels can cause environmental problems. Carbohydrate in banana peel can be utilized as substrate to produce citric acid through fermentation. Citric acid is an...
Activity Enhancement of Antioxidant Contained in Sugar Palm Fruit (Arenga pinnata Merr) Through Solid State Fermentation by Aspergillus oryzae
Merry Meryam Martgrita, Roga Florida Kembaren, Adelina Manurung, Herti Novalia Hutapea, Theodora Mega Putri Lumbangaol
It has been widely known that sugar palm fruit (Arenga pinnata Merr) contains galactomannan that has antioxidant activity. Antioxidants are compounds that can inhibit free radical formation during oxidation reactions in the body that can cause disease. Antioxidant compounds are commonly found in natural...
Electroelution of 31 kDa Immunogenic Protein Fraction from the Salivary Gland of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
Ilma Zakiyyah, Linda Dwi Santika, Syubbanul Wathon, Kartika Senjarini, Rike Oktarianti
Dengue Hemorrhagic Fever (DHF) is a disease generated by dengue virus infection. The dengue virus is transmitted to the host by mosquito vectors. The main and secondary vectors are Aedes aegypti and Aedes albopictus. Dengue virus transmission is mediated by salivary gland proteins that facilitate the...
The Effect of Dietary Bromelain Enzyme on Broiler Chicken (Gallus gallus) Growth Performance
Ni’matul Laili Nur Mahfudhoh, Sajidan, Agung Budiharjo
Many studies have reported that the addition of bromelain enzyme and pineapple extract can increase the growth performance of broiler chickens. However, when the bromelain enzyme was applied to meat, it can digest proteins that cause cell damage. In this study, the addition of the bromelain enzyme with...
Amylase Production by Rhizopus oryzae Using Solid State Fermentation with Cassava Solid Waste as Substrate
Merry Meryam Martgrita, Roga Florida Kembaren, Herti Novalia Hutapea, Ivana Sitepu, Evy Enjelina Simanjuntak
Amylase is an enzyme that is used in various industries such as food, paper, and textile industries. Amylase can be produced by microorganisms through fermentation. Rhizopus oryzae is one of microorganisms that can produce amylase through fermentation. Tapioca solid waste has a good potential as a carbon...
In Vitro Cytotoxicity of Gallic Acid Derivatives (Alkyl gallates) Against Breast MCF-7 Cancer Cells
Ade Arsianti, Maya Dorothea, Naura Syafira, Ananda Tony, Anton Bahtiar
Breast cancer is the most common cancer in women. Based on WHO data in 2011, it is estimated that more than 508,000 women worldwide died of breast cancer. In Indonesia, the prevalence of breast cancer is 26.5% of all cancer cases, in which become the second frequent cancer. To date, the treatments for...
The Roles of Genetic and Epigenetic Aspects in Mandibular Prognathism: A Review
Putri Fatimatus Zahro, Francisca Veyta Ayu, Fadli Jazaldi, Elza Ibrahim Auerkari
Individuals with mandibular prognathism (MP) are characterized by a concave facial profile, and in severe cases, an extremely long face. These clinical characteristics mostly result from the extreme forward growth of the mandible, and are more prevalent in Asians than in Caucasians. MP is a multifactorial...
Genetics and Epigenetics Aspects of Thalassemia
Inayu Mahardhika Putri, Ferry P. Gultom, Elza Ibrahim Auerkari
Hemoglobin contains of heme, which binds iron and protoporphyrin, and globin, which consist of combination of α, β, δ, and γ chain. α-globin globin gene is located in 16p13.3 cytogenetically, and β-globin gene is in 11p15.4. Thalassemia is a blood disorder characterized in the lack or absence of one...
The Comparison of Essential Oil Extraction from Citronella Grass (Cymbopogon nardus L.) Using Solvent-Free Microwave Extraction and Microwave Hydrodistillation Methods
Ditta Kharisma Yolanda Putri, Ardetha Titarnia Aurly, Siti Fatimah, Boy Arief Fachri
Indonesia is a country that has 40–50 types of essential oil-producing plants out of a total of 80 types of essential oils that enter the world market. There are several essential oil-producing plants that until now have not been used optimally. However, the extraction method of citronella oil in Indonesia...
The Effects of Ethanol Extract of Asian Pigeon Wings (Clitoria ternatea L.) Flower on Body Weight and Malondialdehyde Level in Diabetes Rat Model
Tantri Febriana Putri, Brian Wasita, Dono Indarto
Diabetes mellitus. is ai global metabolici disease with high morbidity and imortality rates. Many diabetes patients have difficulties lowering their blood glucose levels although they are taking anti-diabetes drugs. Flower of Asian pigeon wings (Clitoria ternatea L) contains flavonoid, anthocyanin, compounds...
Modifying High Sucrose Tomatoes by Genome Editing A-Review
Muhammad Mufarrij Fuad Ulfi, Ridlo Firmansyah, Wahyu Indra Duwi Fanata, Dibyajyoti Pramanik, Jae-Yean Kim, Sholeh Avivi
Sucrose which is the main product of photosynthesis in plants is resulted from carbon assimilation. The way to produce more sucroses as the sink product has been done a lot recently. However, the way such as overexpression of SPS or harvest environmental engineering needed a lot of samples and a certain...
Patau Syndrome: Genetic and Epigenetic Aspects
Yesi Octavia, Muhammad Garry Syahrizal Hanafi, Fadli Jazaldi, Elza Ibrahim Auerkari
Patau syndrome, also known as trisomy 13 syndrome, is a syndrome caused by a chromosomal abnormality. This rare syndrome is a lethal disease, where the life expectancy more than 1 year after birth is only less than 15%. The cause of Patau Syndrome is an abnormality on chromosome 13, where the possibilities...
Anticancer Effect of Red Fruit Fractions Toward Breast Cancer in T47D Cell and Oral Squamous Cancer in KB Cell
Hana Ratnawati, Yoki Chandra, Endry Kho
Indonesia is one of the mega-biodiversity countries, with many plant species that are useful as medicine. Red Fruit (Pandanus conoideus Lam), found in Jayawijaya, Papua, is empirically believed to have numerous health benefits due to having a huge amount of bioactive compounds, especially carotenoids....
Screen-Printed Carbon Electrode Fabrication Method for Electrochemical Biosensor Application
Eduardus Ariasena, Ivandy Arifin Putra Noerrizky, Raih Rona Althof, Isa Anshori
Screen-printed carbon electrode (SPCE) is one type of electrode commonly used for electrochemical biosensor application. It is fabricated using a screen-printing method, a method in which conductive ink is piled up layer-by-layer using some screen frames. SPCE combines three main electrodes used in the...
Immunogenic Proteins from Salivary Gland of Potential Malaria Vector An. vagus and An. sundaicus
Ika Wahyuni, Rike Oktarianti, Syubbanul Wathon, Lailly Nur Uswatul Hasanah, Kartika Senjarini
The salivary gland proteins of the Anopheles (An.) female mosquito play vital roles in the transmission of Plasmodium into the human host. A comprehensive understanding of Anopheles identity and its related salivary proteins is a key for vector-based malaria control. This research aims to analyse the...
Identification of Protein Levels as Production of Bacteriosin from Lactobacillus Plantarum in Fermented Chicken Eggs
Azmi Mangalisu, Irma Isnafia Arief, Andi Kurnia Armayanti, Zakiah Wulandari
Lactobacillus plantarum is a lactic acid bacterium that can produce lactic acid as well as degrade proteins into soluble proteins, peptides, and amino acids. L. plantarum can also produce bacteriocin, which has antibacterial properties. The goal of this study was to determine the protein levels in fermented...
Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment
Yuliana Heri Suselo, Dono Indarto, Brian Wasita, Hartono
Mirabilis jalapa is a medicinal plant, which is widely distributed in some tropical countries including in Indonesia. Indicaxanthin, Miraxanthin and Boeravinone F active compounds, which are the family member of Betaxanthins in the M. jalapa plant, are able to inhibit hepcidin and to activate matriptase-2...
Changing of Morphological, Anatomical, Cytological Characteristic and Artemisinin Content in Artemisia cina by Colchicine Treatment
Maria Marina Herawati, Endang Pudjihartati, Andree Wijaya Setiawan
The induction of artificial polyploidy has been considered a valuable tool that can improve many plants’ genetics and change their morphological, anatomical, and physiological characteristics. Artemisia cina plants’ polyploidy is induced through shoot culture to increase artemisinin content. This research...
The Effect of Extract Areca Seeds (Areca catechu L.) on the Thickness of the Colonic Tunica Muscularis in Mice (Mus musculus) Feeded Trichuris muris Infective Eggs Peroral
Endy Juli Anto
One species of worm Trichuris trichiura that is often used in research on experimental animals is Trichuris muris. Administration of T. muris infective eggs can significantly increase the thickness of the tunica muscularis of the colon. This thickening of the tunica muscularis intestine is associated...
Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method
Sri Rulianah, Prayitno, Carita Ayu Maulidina
Bagasse is one of the solid wastes from sugar factories which contains lignocellulose. Lignocellulose contains high levels of cellulose so that it has the potential as raw material to produce bioethanol. The crude cellulase produced by Phanerochaete chrysosporium has enzymes capable of degrading lignin,...
Determination of Salinity Tolerance on Cayenne Genotypes Based on Leaf Damage Symptoms
Rustikawati, Mimi Sutrawati, Wuri Prameswari, Catur Herison, Yoga Suprimansyah
Capsicum sp. Has been reported as a salt-sensitive crop. Therefore, using salt-tolerant varieties will be a wise solution for maintaining crop production in coastal areas. Source of saline-tolerance controlling genes are needed to develop saline-tolerant genotypes. The objective of this study was to...
Selection of Potential Plants as Phytoremediation for Heavy Metals in Estuarine Ecosystem: A Systematic Review
Alfin Fatwa M. Afifudin, Rony Irawanto, Neny Purwitasari
The estuary area is a semi-enclosed water body area that borders the sea but still connected to fresh water from rivers. As an area that connects between sea and rivers, the estuary is an area that threatened by human activities, including water pollution of heavy metals. Therefore, it is necessary to...
Genetic and Epigenetic Aspects of Amelogenesis Imperfecta and Dentinogenesis Imperfecta
Nurulia Januarti, Francisca Veyta Ayu, Ria Puspitawati, Elza Ibrahim Auerkari
Amelogenesis imperfecta (AI) and dentinogenesis imperfecta (DGI) are hereditary dental disorders. AI is a developmental condition resulting in enamel defects of all or almost all teeth. The prevalence ranges from 1:700 to 1:14,000 for AI and from 1:6000 to 1:8000 for DGI. DGI is a collective of autosomal...
Isolation and Identification of Biogas-Producing Methanogenic Bacteria from Cow Manure
Grace Roma Artha Samosir, Ellyas Alga Nainggolan, Meiyer Marthen Kinda, Dedy Anwar
Biogas is a methane-rich gas produced from microbial digestion of waste (agricultural, sewage, and landfill) which can be used for power production. The low rate of biogas production from an anaerobic digester becomes a problem in cow manure processing increasing. Biogas production is influenced by the...
Molecular Aspects of Systemic Lupus Erythematosus
Benita Kurniawan, Francisca Veyta Ayu, Benny Mulyono Soegiharto, Elza Ibrahim Auerkari
Systemic lupus erythematosus (SLE) is an autoimmune disease caused by combined genetic and environmental factors that change the normal pathways of human immunity. SLE is potentially fatal and carries significant morbidity. The SLE patient can inherit multiple predisposing genes. T The identified SLE...
An Extracellular Cellulase Production Under Solid-State Fermentation of Coffee Pulp Waste by Aspergillus sp. VTM1 and Its Purification
Ramdhan Putrasetya, Reni Rusdianti, Viara Septaninda Sugianto, Rudju Winarsa, Siswoyo, Kahar Muzakhar
Microbial utilization of agricultural biomass wastes has been widely used to synthesize certain an enzyme. We previously investigated that cellulase can be produced under solid stated fermentation based on coffee pulp waste substrate using Aspergillus sp. VTM1. Crude cellulase production was optimized...
Production and Partial Purification of Cellulase from Aspergillus sp. VT12 by Solid-State Fermentation Using Coffee Pulp
Farah Salma Elida, Dwi Fajarwati Ramadhani, Rudju Winarsa, Hidayat Teguh Wiyono, Siswoyo, Kahar Muzakhar
Cellulase has been widely used in many applications of industries and is commonly produced by a cellulase-producing-fungus using production substrates from agricultural wastes biomass. In the wet processing of the coffee, up to 63% of coffee pulp rich-cellulose has been released. This potential waste...
The Cytotoxicity Effect of Ethanol Extract and Alkaloid Fraction of Mirabilis jalapa Leaves in Hepatocarcinoma Cell Line
Yuliana Heri Suselo, Dono Indarto, Brian Wasita, Hartono
Mirabilis jalapa is a dry tropical plant which is well grown in Indonesia. Betaxanthin active compound is present in the M. jalapa plant, which plays a role in iron metabolism. Cytotoxicity activity is one important assay for drug development. Therefore, this study aimed to determine whether ethanol...
Pectinase Production by Aspergillus VTM4 Induced by Pomelo Pulp (C. maxima Merr.) As Substrate
Rudju Winarsa, Okta Novalia Gasani, Tamimul Badriya, Hidayat Teguh Wiyono, Siswoyo, Kahar Muzakhar
Pectinase is a heterogeneous enzyme that hydrolyzes pectin, which is currently in high demand in the industry, so a strategy is needed to produce it economically. Aspergillus VTM4 is a mold that can grow on pomelo pulp and produce pectinase. Under solid-state fermentation, the crude pectinase was produced...
Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification
Azizah, Atim Ainul Hidayah, Rosa Amelia, Hidayat Teguh Wiyono, Siswoyo, Kahar Muzakhar
Hydrolytic Pectinase is an important enzyme and is widely used in several industries. In the previous investigation, this enzyme could be produced by Aspergillus sp. VTM5 under solid-state fermentation and the coffee pulp waste substrates were used. The specific activity of about 0.4 U/ml of pectinase...
Coffee Pulp Waste Substrate Based in Cellulase Production by Penicillium sp. VT11 Under Solid-State Fermentation
Hidayat Teguh Wiyono, Nabilah Ilmalah Sunarto, Finda Rahmawati, Rudju Winarsa, Siswoyo, Kahar Muzakhar
Coffee pulp is an agro-industrial waste that is produced in huge amounts in Indonesia. The coffee pulp contains hemicellulose, with 63% of the total cellulose. In our previous study, the cellulase activity of Penicillium sp VT11 from coffee pulp waste was assayed under the solid-state fermentation (SSF)...
Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate
Trianawati, Rudju Winarsa, Siswoyo, Kahar Muzakhar
Cellulase can be produced by a microorganism, such as fungi. The cellulolytic microorganism used in this study was Paecilomyces lilacinus ICP1. P. lilacinus ICP1 is one of the indigenous fungi from coffee pulp that has been known to produce cellulose. Coffee pulp is expected to be able to replace the...
Isolation and Identification of Hemicellulolytic Bacteria from Indonesian Coffee Pulp Waste
Sattya Arimurti, Yulia Nuraini, Tri Ardyati, Suharjono Suharjono
Coffee pulp waste contains 25.9–33.0% hemicellulose. Hemicellulolytic bacteria possess the ability to depolymerize hemicellulose by producing hemicellulase enzymes. The objective of the research is to isolate and identify the hemicellulolytic bacteria from Indonesian coffee pulp waste. Hemicellulolytic...
Linker Optimization in Breast Cancer Multiepitope Peptide Vaccine Design Based on Molecular Study
Fadilah Fadilah, Rafika Indah Paramita, Linda Erlina, Khaerunissa Anbar Istiadi, Puspita Eka Wuyung, Aryo Tedjo
Breast cancer is most common cancer diagnosed in women. The urgency of developing effective therapeutic approaches is needed, both passive and active immunotherapy using vaccines. Immunoinformatics approach for epitope prediction of cancer proteins is one of promising approach in peptide vaccine development....
Phytochemical Screening and Antimicrobial Activity of Cordyline fruticosa Leaf Infusion and Ethanol Extract Against Shigella dysentriae and Candida albicans
Vilya Syafriana, Amelia Febriani, Hera Ratnasari
Cordyline fruticosa, also known to Indonesians as andong, is one of the simplest plants to grow. This plant is commonly used as an ornamental plant in yards, gardens, cemeteries, as well as a road barrier. Although it is primarily grown for ornamental purposes, the leaves of this plant empirically have...
Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation
Esti Utarti, Annisa’ul Jannah, Sattya Arimurti
Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production...
Susceptibility Status of Culex quinquefasciatus to Malathion in Brebes Regency, Indonesia
Husnatun Nihayah, Budi Mulyaningsih, Sitti Rahmah Umniyati
Brebes Regency is a bancroftian filariasis endemic area. Culex quinquiefasciatus mosquito is accused as a vector transmitting bancroftian filariasis. The eradication of disease vector that has been widely performed in Indonesia relies on the use of malathion insecticide. The use of such insecticide in...
The Comparative Effects of Branded and Local High Fat Foods on Body Mass Index and Vascular Wall Thickness in Male Wistar Rats for Development of Atherosclerosis Animal Model
Prasetyo Sarwono Putro, Dono Indarto, Bambang Purwanto, Widiastuti Soewondo, Sulistyani Kusumaningrum
Cardiovascular diseases are the leading cause of mortality over the world, which is approximately 17.9 million patients’ death in 2019 or 32% of all deaths worldwide. Atherosclerosis formation due to accumulated plaques in the tunica intima of vascular walls underlies the pathogenesis of cardiovascular...
The Influence of Gum Inducer Solution Administration on the Gum Production of the Jaranan Plant (Lannea coromandelica (Houtt.) Merr.)
Hidayat Teguh Wiyono, Selin Monika Prihasinta, Dwi Setyati, Nadhea Ayu Sukma
Jaranan plant (Lannea coromandelica) grows massively in the buffer zone and utilization zone of Baluran National Park. This plant produces gum in branches and stems. Local people extract gum by cutting down stems as medicine for diarrhea, typhus, and eye infections treatment. The study aims to test gum...
In Silico Study of Antigenicity and Immunogenicity of the D7 Protein from Salivary Glands of Aedes aegypti
Kartika Senjarini, Susmaya Atmandaru, Ari Satia Nugraha, Syubbanul Wathon, Rike Oktarianti
The Aedes aegypti mosquito has been known as the primary vector in the transmission of Dengue Hemorrhagic Fever (DHF). D7 protein has been reported as a protein expressed in the salivary glands of mosquito vectors that can increase the transmission of the pathogen because of its ability to immunosuppress...
DNA Barcoding of Vanda tricolor Lindl. Based on matK, rbcL and ITS2 Sequences
Mukhamad Su’udi, Waki’atil Rosida, El Shania Ravitamala, Dwi Setyati
Vanda tricolor Lindl. is one of the important ornamental plants favored by many people in the world. However, this plant required many years for vegetative growth before the first flowers emerge. Since the flower is one of the most important organs in morphological identification, an alternative approach...
Ecological Value of Tree Vegetation at Erek-erek Biosite of Ijen Geopark, Indonesia
Hari Sulistiyowati, Arif Mohammad Siddiq, Abdillah Baraas, Fikli Perdana Kusuma, Firman Syauqi Nur Sabila
Erek-erek is Ijen Geopark Biosite located in an area above 1000m altitude. It is a primary tropical rain forest ecosystem in a quarter volcano which is already in the stage of climax succession because the canopy stratification is very complex. However, there is limited information on the tree vegetation...