Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

DOI

10.2991/978-94-6463-062-6_40How to use a DOI?

Keywords

Bagasse; Bioethanol; Crude cellulase; Phanerochaete chrysosporium; Saccharomyces cerevisiae

Abstract

Bagasse is one of the solid wastes from sugar factories which contains lignocellulose. Lignocellulose contains high levels of cellulose so that it has the potential as raw material to produce bioethanol. The crude cellulase produced by Phanerochaete chrysosporium has enzymes capable of degrading lignin, cellulose and hemicellulose simultaneously. The aim of the research is to analyses the effect of adding crude cellulose and Saccharomyces cerevisiae to the yield and concentration of bioethanol. The research was conducted by drying and reducing the size of the bagasse, cultivating of the Phanerocheate chrysoporium, fermenting the bagasse, and filtering. The resulting crude cellulase activity will be tested using the DNS method. The best crude cellulase activity is used to make bioethanol from bagasse as raw material. Simultaneous Saccharification and Fermentation (SSF) method is used in the production of bioethanol. The concentration of the bioethanol product was analysed using Gas Chromatography. The variables used are the addition of 6% bagasse, crude cellulose 40, 50, and 60% (v/v), Saccharomyces cerevisiae 1, 2, 3, 4, and 5% (w/v). In this study, the best conditions were obtained by adding 60% crude cellulase and 4% Saccharomyces cerevisiae, obtained bioethanol with a concentration of 9.18%, and a yield of 16.79%.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Sri Rulianah
AU  - Prayitno
AU  - Carita Ayu Maulidina
PY  - 2022
DA  - 2022/12/22
TI  - Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 399
EP  - 408
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_40
DO  - 10.2991/978-94-6463-062-6_40
ID  - Rulianah2022
ER  -