Analysis of Amino Acids, Protein Profile, Calcium and Phosphorus Levels of Upeneus moluccensis Waste (Thorns and Scales)

DOI

10.2991/978-94-6463-062-6_11How to use a DOI?

Keywords

Amino acid; protein; Calsium; Phosphorus; Upeneus moluccensis

Abstract

Upeneus molucensis is seawater fishes that are consumed by many people so it leaves a lot of waste (including scales and thornss). On the other hand, the utilization of fish waste is still not much. Whereas, waste contains a lot of amino acids, protein, Calcium and Phosphorus. This study analyzed amino acids, protein profiles, levels of Calcium and Phosphorus in the thornss and scales of U. moluccensis. Analyze of amino acids used HPLC: The samples were injected into the HPLC column, waiting until the separation of all amino acids were complete. The amino acids standard used a mixed standard consisting of 15 types of amino acids. Protein analyzes used SDS-PAGE: 15% acrylamide, 50-95 V for 5 h in electoda buffer (glycine 192mM, Trisbase 25mM, SDS0, 1%). Staining used Coomassie Brilliant Blue. While the analysis of Calcium and Phosphorus using Atomic Absorption Spectrophotometer (AAS) and Spectrophotometer UV-Vis. The sample was added with nitric acid, HClO4:HNO3, distilled water and HCl, heated and filtered. Statistical analysis was carried out descriptively (amino acids and protein profiles), T test (Calcium and Phosphorus level). U. moluccensis amino acids found 12 amino acids there are 5 types of essential amino acids and 7 non-essential. Protein profiles of scales were 11 bands, thorns 13 bands. Calcium level (ppm) of U. molucensis (scales 3227, thorns 2782), Phosphorus level (scales 16514, thorns 23082). The scales and thorns of U. moluccensis fish were found in various types of amino acids, proteins profiles with various molecular weights and higher Calcium content were found in scales, but higher phosphorus was found in thornss.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - I Dewa Ayu Ratna Dewanti
AU  - I Dewa Ayu Susilawati
AU  - Pujiana Endah Lestari
AU  - Erawati Wulandari
AU  - Ristya Widi Endah Yani
AU  - Sunlip Wibisono
PY  - 2022
DA  - 2022/12/22
TI  - Analysis of Amino Acids, Protein Profile, Calcium and Phosphorus Levels of Upeneus moluccensis Waste (Thorns and Scales)
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 98
EP  - 109
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_11
DO  - 10.2991/978-94-6463-062-6_11
ID  - Dewanti2022
ER  -