Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment

DOI

10.2991/978-94-6463-062-6_37How to use a DOI?

Keywords

Mirabilis jalapa leaves; Betaxanthin; Extraction and fractionation; Iron metabolism

Abstract

Mirabilis jalapa is a medicinal plant, which is widely distributed in some tropical countries including in Indonesia. Indicaxanthin, Miraxanthin and Boeravinone F active compounds, which are the family member of Betaxanthins in the M. jalapa plant, are able to inhibit hepcidin and to activate matriptase-2 for increase of iron absorption in silico. Meanwhile, Betalains compound belongs to alkaloid compound, which is the precursor for Betaxanthin and Betacyanin synthesis. Therefore, this study aimed to identify Betaxanthin, Betacyanin and Betalain compounds in extract and fraction of M. jalapa leaves. Dried M. jalapa leaves were purchased from Merapi Farma, Yogyakarta. Extraction and fractionation of M. jalapa leaves were carried out using maceration and physical methods respectively. Alkaloid compounds in ethanol extract and alkaloid fraction were identified using a thin layer chromatography with piperine as the standard compound. Betaxanthin, Betacyanin and Betalain levels were measured using the UV-Vis spectrophotometry. From M. jalapa simplicia, it yielded only 7.7% ethanol extract while we got 41.33% alkaloid fraction. Alkaloid compounds were only detected in M. jalapa leaves fraction instead of the ethanol extract. Betaxanthin levels in alkaloid fraction were 23 times higher than that of the ethanol extract. Higher Betaxanthin levels were also observed in ethanol extract, compared to Betacyanin and Betalain levels. The alkaloid fraction of M. jalapa leaves is a better source of Betaxanthin compound and its derivatives than the ethanol extract. Further investigation is needed to determine phytochemicals compounds in the alkaloid fraction of M. jalapa leaves and their role in iron metabolism.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Yuliana Heri Suselo
AU  - Dono Indarto
AU  - Brian Wasita
AU  - Hartono
PY  - 2022
DA  - 2022/12/22
TI  - Alkaloid Fraction of Mirabilis Jalapa Leaves has Higher Betaxanthin Levels than Ethanol Extract and is Potentially Developed for Anemia Treatment
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 370
EP  - 377
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_37
DO  - 10.2991/978-94-6463-062-6_37
ID  - Suselo2022
ER  -