Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation

Authors
Esti Utarti1, *, Annisa’ul Jannah1, Sattya Arimurti1
1Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
*Corresponding author. Email: esti.fmipa@unej.ac.id
Corresponding Author
Esti Utarti
Available Online 22 December 2022.
DOI
10.2991/978-94-6463-062-6_56How to use a DOI?
Keywords
CMCase; Actinomycetes; Nicotiana tabacum L.; Submerged fermentation
Abstract

Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production by three actinomycetes with the best CMCase activity index using tobacco stalk as substrate through submerged fermentation, and morphology identification actinomycetes with the highest CMCase activity. A total of 71 actinomycetes have been isolated from the rhizosphere of tobacco plant. In this study, the ability of actinomycetes to produce cellulase through submerged fermentation using tobacco stalk substrate powder. There were about 71 actinomycetes isolated and all of them have cellulolytic activities. Based on the screening of cellulolytic activity on CMC (carboxymethyl cellulose) agar plate, the enzyme activity index of ATG 38 isolate was higher than others, with 2.50 ± 0.26 respectively. The cellulase production using tobacco stalk as substrate through submerged fermentation was optimal showing that CMCase production of actinomycete ATG 38 isolates maximal after four days incubation with 0,40 mU/mL enzyme activity. Microscopic morphological observations showed that actinomycete ATG 38 isolate belongs to the genus Streptomyces.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 December 2022
ISBN
10.2991/978-94-6463-062-6_56
ISSN
2468-5747
DOI
10.2991/978-94-6463-062-6_56How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Esti Utarti
AU  - Annisa’ul Jannah
AU  - Sattya Arimurti
PY  - 2022
DA  - 2022/12/22
TI  - Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 550
EP  - 559
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_56
DO  - 10.2991/978-94-6463-062-6_56
ID  - Utarti2022
ER  -