Susceptibility Status of Culex quinquefasciatus to Malathion in Brebes Regency, Indonesia

DOI

10.2991/978-94-6463-062-6_57How to use a DOI?

Keywords

Indonesia; Cx. quinquefasciatus; Malathion; Non-specific esterase enzyme

Abstract

Brebes Regency is a bancroftian filariasis endemic area. Culex quinquiefasciatus mosquito is accused as a vector transmitting bancroftian filariasis. The eradication of disease vector that has been widely performed in Indonesia relies on the use of malathion insecticide. The use of such insecticide in the long term might decrease susceptibility status for the targeted vector. The determination on the susceptibility status in Cx. quinquefasciatus in Brebes Regency should be pursued to monitor the insecticide use. The research was to elucidate the susceptibility status of Cx. quinquefasciatus on malathion insecticide and to review the activity of non-specific esterase enzyme Cx. quinquefasciatus that have been collected from endemic (Ketanggungan district) and nonendemic (Paguyangan district) bancroftian filariasis in Brebes, Central Java, Indonesia. The determination on the susceptibility status was performed by CDC bottle bioassay method on malathion with the diagnostic dose 400 μg/bottle. Then, the biochemical assay was conducted by performing Lee method with substrate α-naphthyl acetate to test the presence of activity on non-specific esterase enzymes. Culex quinquefasciatus mosquitos that had been collected from endemic area and non-endemic area were still susceptible to malathion; percentage of mortality is 100%. In addition, the biochemical assay showed increasing activity on non-specific esterase enzymes on both locations. This preliminary research reflects that there is decomposition of insecticides that enter the body which may lead to resistance. Therefore, further molecular research needs to be done to determine genetic mutation in Cx. quinquefasciatus in Brebes.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Husnatun Nihayah
AU  - Budi Mulyaningsih
AU  - Sitti Rahmah Umniyati
PY  - 2022
DA  - 2022/12/22
TI  - Susceptibility Status of Culex quinquefasciatus to Malathion in Brebes Regency, Indonesia
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 560
EP  - 571
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_57
DO  - 10.2991/978-94-6463-062-6_57
ID  - Nihayah2022
ER  -