Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Humoral Immune Response (IgG) of BALB/c Mice (Mus musculus) Post-injection by 56 kDa Immunogenic Protein Extract from the Salivary Glands of Aedes aegypti L.

Authors
Aisyah1, Rike Oktarianti1, *, Kartika Senjarini1, Syubbanul Wathon1
1Biology Department, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
*Corresponding author. Email: rike.fmipa@unej.ac.id
Corresponding Author
Rike Oktarianti
Available Online 22 December 2022.
DOI
10.2991/978-94-6463-062-6_16How to use a DOI?
Keywords
56 kDa immunogenic protein; Salivary gland; Ae. aegypti; IgG
Abstract

The primary mosquito vector of dengue virus is Ae. aegypti, and dengue virus transmitted by saliva mosquito’s during blood feeding. The saliva composed of numerous proteins with capacity to disrupt hemostasis or modulate the host immune response. The previous study identified two immunogenic proteins in the salivary glands of Ae. aegypti i.e. 56 kDa and 31 kDa. However, the mechanism of host’s humoral immune response (IgG) against the 56 kDa immunogenic protein is not known. Therefore, this study was conducted to observe the mice strain BALB C immune response (IgG) against the 56 kDa. The mice were divided into three groups of injection treatments, particularly Group-A (Tris-Cl 0.05M), Group-B (adjuvant), and Group-C (56 kDa immunogenic protein 0.1 µg/µL + adjuvant). Injection was done every two weeks in total six weeks of treatment. The IgG level was measured every two weeks using ELISA method. The study results exhibited the Group-C had higher IgG level than Group-A and Group-B. The IgG level of Group-C gradually increased along the increasing of 56 kDa protein exposures, meanwhile the IgG level of Group-A and Group-B tended to be steady before and after treatment. These results suggested that repeated exposure of 56 kDa immunogenic protein elevated the humoral immune response (IgG) in host.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 December 2022
ISBN
10.2991/978-94-6463-062-6_16
ISSN
2468-5747
DOI
10.2991/978-94-6463-062-6_16How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Aisyah
AU  - Rike Oktarianti
AU  - Kartika Senjarini
AU  - Syubbanul Wathon
PY  - 2022
DA  - 2022/12/22
TI  - Humoral Immune Response (IgG) of BALB/c Mice (Mus musculus) Post-injection by 56 kDa Immunogenic Protein Extract from the Salivary Glands of Aedes aegypti L.
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 157
EP  - 167
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_16
DO  - 10.2991/978-94-6463-062-6_16
ID  - 2022
ER  -