In Vitro Analysis of Human IgG Immune Response Against 31 kDa and 67 kDa Immunogenic Protein from Aedes albopictus Salivary Glands
- DOI
- 10.2991/978-94-6463-062-6_13How to use a DOI?
- Keywords
- Absorbance; IgG; Immunogenic; Protein
- Abstract
Mosquito salivary glands contain protein substances that can facilitate dengue virus transmission. Those proteins have abilities to induce an immune response and specific antibody production. Previous studies showed that 31 kDa and 67 kDa protein fractions from Aedes albopictus salivary glands are immunogenic protein. They can be recognized by the human IgG antibodies of endemic residents from Jember Regency by the Western Blot analysis. However, the IgG levels are not known quantitatively. Therefore, this study is expected to decide the level of IgG as a human humoral immune response against 31 kDa and 67 kDa protein fractions from Ae. Albopictus salivary glands. We separated salivary gland proteins using SDS-PAGE and continued using the electroelution method to get purified protein fraction. These proteins then cross-reacted with human blood serum using Indirect ELISA. The results showed that the IgG level against 31 kDa protein fraction based on the absorbance value in the healthy human population, DHF patients, and neonates were 0.229, 0.198, 0.151 respectively in individual serum and 0.229, 0.198, 0.151 in pool serum. While the IgG level against 67 kDa protein fraction based on the absorbance values in the individual serum of healthy human population, DHF patients, and neonates were 0.4293, 0.3159, 0.2997 respectively and 0.410, 0.096, 0.037 in pool serum. Those results indicate that the IgG antibodies of each population group can well recognize the 31 kDa and 67 kDa protein fractions. The highest levels of IgG found in healthy residents, followed by DHF patients, and neonates.
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- © 2023 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Syubbanul Wathon AU - Izza Afkarina AU - Unzilatir Rohmah AU - Rike Oktarianti AU - Kartika Senjarini PY - 2022 DA - 2022/12/22 TI - In Vitro Analysis of Human IgG Immune Response Against 31 kDa and 67 kDa Immunogenic Protein from Aedes albopictus Salivary Glands BT - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021) PB - Atlantis Press SP - 122 EP - 134 SN - 2468-5747 UR - https://doi.org/10.2991/978-94-6463-062-6_13 DO - 10.2991/978-94-6463-062-6_13 ID - Wathon2022 ER -