Coffee Pulp Waste Substrate Based in Cellulase Production by Penicillium sp. VT11 Under Solid-State Fermentation

DOI

10.2991/978-94-6463-062-6_51How to use a DOI?

Keywords

Cellulase; Coffee pulp; Penicillium sp. VT11; Solid-state fermentation

Abstract

Coffee pulp is an agro-industrial waste that is produced in huge amounts in Indonesia. The coffee pulp contains hemicellulose, with 63% of the total cellulose. In our previous study, the cellulase activity of Penicillium sp VT11 from coffee pulp waste was assayed under the solid-state fermentation (SSF) method. In this study, we increased the production of the enzyme using the SSF method and purified it using dialysis and ion-exchange chromatography. The optimum cellulase activity was 0.8 U/ml after four days of incubation at 30 ℃. The enzyme after dialyzed using 12–14 kDa pores cellulose membrane resulted in 2.25 times increase in activity of 0.78 U/ml. The results proved that Penicillium sp. VT11 can utilize the coffee pulp waste as carbon and nitrogen for its growth and produce cellulose. The dialyzed enzyme was loaded into open column DEAE Cellulose DE-52 chromatography with gradient 0–0.6 of NaCl which two peaks of cellulase activity were detected. The peaks were eluted in 0.2 M of NaCl with cellulase activity of 0.14 U/ml and 0.5 M of NaCl with 0.156 U/ml cellulose activity respectively. The 20 mM acetate buffer pH 5 was applied for the dialysis, cellulase assay, and purification processes stated above. There were two peaks found, indicating that two types of cellulases were produced during solid-state fermentation. Molecular analysis is required to distinguish between these two forms of cellulases. This article details all of the research methods.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Hidayat Teguh Wiyono
AU  - Nabilah Ilmalah Sunarto
AU  - Finda Rahmawati
AU  - Rudju Winarsa
AU  - Siswoyo
AU  - Kahar Muzakhar
PY  - 2022
DA  - 2022/12/22
TI  - Coffee Pulp Waste Substrate Based in Cellulase Production by Penicillium sp. VT11 Under Solid-State Fermentation
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 501
EP  - 509
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_51
DO  - 10.2991/978-94-6463-062-6_51
ID  - Wiyono2022
ER  -