Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Pectinase Production by Aspergillus VTM4 Induced by Pomelo Pulp (C. maxima Merr.) As Substrate

Authors
Rudju Winarsa1, Okta Novalia Gasani1, Tamimul Badriya1, Hidayat Teguh Wiyono1, Siswoyo2, Kahar Muzakhar1, *
1Department of Biology, Faculty of Mathematics and Natural Sciences, Jember University, Jember, Indonesia
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jember University, Jember, Indonesia
*Corresponding author. Email: kaharmzk@unej.ac.id
Corresponding Author
Kahar Muzakhar
Available Online 22 December 2022.
DOI
10.2991/978-94-6463-062-6_49How to use a DOI?
Keywords
Aspergillus sp. VTM4; Pectin-rich pomelo pulp; Pectinase
Abstract

Pectinase is a heterogeneous enzyme that hydrolyzes pectin, which is currently in high demand in the industry, so a strategy is needed to produce it economically. Aspergillus VTM4 is a mold that can grow on pomelo pulp and produce pectinase. Under solid-state fermentation, the crude pectinase was produced with the activity of 1.6 U/ml when 10 gr of dried pomelo pulp was inoculated by 107 spores of Aspergillus sp. VTM4 and incubated for 48 h at 30 ℃. After dialysis using a cellulose membrane tube 12–14 kDa, the pectinase activity was increased by 0.182 U/ml. Further purification step on the DEAE-Cellulose DE-52 open column with a gradient of NaCl 0–0,6M in 20 mM acetate buffer pH 5 was carried out. The peak fraction as pectinase activity increased to 0.369 U/ml with 1,516 purification fold and 5,3% yield. Based on these results, it can be proved that pomelo pulp can be used as a substrate for pectinase production by Aspergillus VTM4. Further research to analyze the properties and characterize the stability of pectinase against temperature, pH, the effect of heavy metals, and molecular analysis is needed. Complete steps and results of this investigation are discussed in this paper.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 December 2022
ISBN
10.2991/978-94-6463-062-6_49
ISSN
2468-5747
DOI
10.2991/978-94-6463-062-6_49How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Rudju Winarsa
AU  - Okta Novalia Gasani
AU  - Tamimul Badriya
AU  - Hidayat Teguh Wiyono
AU  - Siswoyo
AU  - Kahar Muzakhar
PY  - 2022
DA  - 2022/12/22
TI  - Pectinase Production by Aspergillus VTM4 Induced by Pomelo Pulp (C. maxima Merr.) As Substrate
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 482
EP  - 491
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_49
DO  - 10.2991/978-94-6463-062-6_49
ID  - Winarsa2022
ER  -