Isolation and Identification of Hemicellulolytic Bacteria from Indonesian Coffee Pulp Waste

Sattya Arimurti; Yulia Nuraini; Tri Ardyati; Suharjono Suharjono

doi:10.2991/978-94-6463-062-6_53

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Isolation and Identification of Hemicellulolytic Bacteria from Indonesian Coffee Pulp Waste

Authors

Sattya Arimurti¹^{, *}, Yulia Nuraini³, Tri Ardyati², Suharjono Suharjono²

¹Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia

²Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia

³Department of Soil Sciences, Faculty of Agriculture, Brawijaya University, Malang, Indonesia

^*Corresponding author. Email: arimurti.fmipa@unej.ac.id

Corresponding Author

Sattya Arimurti

Available Online 22 December 2022.

DOI: 10.2991/978-94-6463-062-6_53 How to use a DOI?
Keywords: Hemicellulolytic bacteria; Coffee pulp waste; Bacillus aureus XAJ3
Abstract: Coffee pulp waste contains 25.9–33.0% hemicellulose. Hemicellulolytic bacteria possess the ability to depolymerize hemicellulose by producing hemicellulase enzymes. The objective of the research is to isolate and identify the hemicellulolytic bacteria from Indonesian coffee pulp waste. Hemicellulolytic bacteria were isolated from coffee pulp wastes of Coffea arabica and C. canephora from two coffee plantations in East Java Province, Indonesia. These isolates were selected based on the hydrolysis of xylan and xylanase activity on the xylan medium. The best isolates were identified based on 16S rDNA sequence similarity. In this research, 23 bacteria had isolated. Five isolates XRM21, XAJ25, XAJ30, XAJ31, and XAJ34 had higher hemicellulolytic activity than others. The XAJ25, XAJ31, and XAJ34 isolates had a good xylanase activity performance. The XAJ34 isolate had the highest xylanase activity (3.38 ± 0.65 U/ml). The XAJ34 isolate based on 16S rDNA sequence similarity was identified as Bacillus aureus. This bacterium is considered a potential indigenous isolate to degrade hemicellulose of coffee pulp waste.
Copyright: © 2023 The Author(s)
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
Series: Advances in Biological Sciences Research
Publication Date: 22 December 2022
ISBN: 10.2991/978-94-6463-062-6_53
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-062-6_53 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Sattya Arimurti
AU  - Yulia Nuraini
AU  - Tri Ardyati
AU  - Suharjono Suharjono
PY  - 2022
DA  - 2022/12/22
TI  - Isolation and Identification of Hemicellulolytic Bacteria from Indonesian Coffee Pulp Waste
BT  - Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)
PB  - Atlantis Press
SP  - 517
EP  - 527
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-062-6_53
DO  - 10.2991/978-94-6463-062-6_53
ID  - Arimurti2022
ER  -

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