Pathological Changes of Fasciola gigantica After Treatment with Nigella sativa in Vitro

DOI

10.2991/absr.k.210420.013How to use a DOI?

Keywords

Herbal anthelmintic property, tegument disruption, Fasciola gigantica, Nigella sativa

Abstract

Black cumin (N. sativa) has been traditionally used as medicine for various infectious and non-infectious diseases, such as skin infection, conjunctivitis, hypertension, gastrointestinal problems, diabetes, and diarrhea. This study aimed at finding out the effect of black cumin, N. sativa on the survival and pathological changes of liver flukes Fasciola gigantica (F. gigantica) in vitro. The seeds of N. sativa were extracted with maceration using methanol. Mature F. gigantica flukes were stored in PBS solution and treated with different concentrations of N. sativa extract namely 10% (T1), 25% (T2), and 50% (T3). Similar experiment using Albendazole 0.24 mg/ml and 1xPBS was used as positive (C1) and negative (C2) controls, respectively. All treatments were done at room temperature. The dead flukes were further examined for histopathological changes. The results showed that 50% concentration of extract killed all flukes within 15 minutes, much faster than caused by albendazole (30 minutes). In T2 (25% extract), all flukes were died in 31.5 minutes. The extract of N. sativa caused severe disruption of tegument, the alteration of internal organs, and the absence of tegument spine. The intestine showed inflammation with severity levels were inline with the extract concentration. There was also disintegration of testicular cells observed in the flukes treated with black cumin. In conclusion, N. sativa seed extract has potential medical property against F. gigantica in vitro.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Muhammad Hambal
AU  - Alfifatul Hidayah
AU  - Ummu Balqis
AU  - Farida Athaillah
AU  - Muttaqien
AU  - Siti Rani Ayuti
AU  - Wahyu Eka Sari
AU  - Henni Vanda
PY  - 2021
DA  - 2021/04/21
TI  - Pathological Changes of Fasciola gigantica After Treatment with Nigella sativa in Vitro
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 56
EP  - 59
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.013
DO  - 10.2991/absr.k.210420.013
ID  - Hambal2021
ER  -