Profile of Antigen Excretory-Secretory Schistosoma Japonicum in the Development of Elisa Method to Detect Schistosomiasis in Indonesia
- DOI
- 10.2991/absr.k.210420.005How to use a DOI?
- Keywords
- Antigen Excretory-Secretory, Schistosoma japonicum, ELISA
- Abstract
The development of ELISA method to detect patients with schistosomiasis in Indonesia is a detection of the antigens that are derived from the peripheral vessels in the blood serum. This research is conducted in the Napu Plateau of Poso Regency, Central Sulawesi, Indonesia. The goal is to develop ELISA method with polyclonal antibodies to detect antigenic-excretory antigen of S. japonicum in patients with schistosomiasis. Activities in the study include activities in the field namely the isolation of worm S. japonicum and activities in laboratories such as production, characterization and purification by Bradford methods as well as electrophoresis test to see the protein Antigen profile. The results of the study of the 30 worm Schistosoma collected from 5 rats suffered schistosomiasis, obtained as much as 42.5 ml with a protein concentration of 1.351 mg/ml, and had two patterns of polypeptide tape with a molecular weight range of 20 and 39 Dalton kilo (kDa). The conclusion of the resulting Antigen ES S. japonicum profile can induce a good polyclonal antibody in the development of ELISA method to detect the AgES in the serum of schistosomiasis.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Samarang AU - Made Agus Nurjana AU - Malonda Maksud AU - Sri Murtini AU - Fadjar Satrija PY - 2021 DA - 2021/04/21 TI - Profile of Antigen Excretory-Secretory Schistosoma Japonicum in the Development of Elisa Method to Detect Schistosomiasis in Indonesia BT - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020) PB - Atlantis Press SP - 20 EP - 24 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210420.005 DO - 10.2991/absr.k.210420.005 ID - 2021 ER -