Identification and Characterization of Protamine 3 (prm3) Gene in Aceh Bull Testis

DOI

10.2991/absr.k.210420.031How to use a DOI?

Keywords

Aceh cow, prm3 gene amplification, DNA sequencing, fertility

Abstract

The incidence of infertility in breeding male cows highly contributes to economic loss in breeders. To date, there is still a lack of a precise and accurate molecular biomarker to predict the fertility rate on each ejaculation. Therefore, it is important to accurately identify genes related to the fertility of breeding male cows to improve the efficiency of cattle reproduction. In cows, the prm1, prm2, prm3, and tnp2 genes encode basic chromosome proteins located in a compact gene cluster observed in rats and humans. Recently, there is only a few studies and information on prm3. Therefore, explanations on its function, gene profile, characteristics, regulation pattern, its correlation to sperm motility, and fertility in male cows are limited. This study aims to identify and characterize protamine 3 derived from Aceh bull testis, using PCR and DNA sequence analysis. This study was conducted on 10 Aceh bull testes obtained from a slaughterhouse. The steps taken were DNA isolation, prm3 gene amplification, DNA sequencing, and analysis of nucleotide sequences. The results showed that the nucleotide sequence of the prm3 gene in Aceh bull had a high level of homology with the prm3 gene from Bos Taurus and Bos indicus after being compared with data from The GenBank which was around 98-99%. From this study, it can be concluded that the prm3 gene from Aceh cow is identical to the prm3 gene from Bos taurus and Bos indicus at the level of DNA sequence.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Rumi Sahara Zamzami
AU  - Muslim Akmal
AU  - Teuku Zahrial Helmi
AU  - Rusli Rusli
AU  - Sugito Sugito
AU  - Tongku Nizwan Siregar
AU  - Sri Wahyuni
PY  - 2021
DA  - 2021/04/21
TI  - Identification and Characterization of Protamine 3 (prm3) Gene in Aceh Bull Testis
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 145
EP  - 148
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.031
DO  - 10.2991/absr.k.210420.031
ID  - Zamzami2021
ER  -