Effect of Veitchia merrillii Extract on Mortality and Tegument Structure of Fasciola gigantica

DOI

10.2991/absr.k.210420.015How to use a DOI?

Keywords

Plant extract, Anthelmintic property, Mortality time, Histopathology examination

Abstract

This study was conducted to explore the effect of methanolic extract of Veithcia merrillii on mortality time and histopathological changes of Fasciola gigantica in vitro. This study used 25 adult flukes of F. gigantica, divided into five groups: C0 as negative control (0.9% PBS), C1 as positive control (0.024mg/ml albendazole), and treatment groups were P1, P2, and P3 used 10%, 25% and 50% extract, respectively. The observation was carried out every 15 minutes to monitor the motility and mortality of each treatment group. Mortality time of each group was determined by score index and analyzed descriptively. Dead flukes from each group were then prepared for histological examination. The results revealed that 50% of V. merrillii extract (P3) had the same mortality time as C1, where all the flukes died after 30 minutes of treatment. Group P2 had a longer mortality time at 45 minutes and P1 was at 75 minutes. Histopathological observation showed alteration on some organs for C1 and treatment groups, including separation of flukes tegument from parenchyma, vacuolization, and disintegration of testis. From the results it can be concluded that methanolic extract of V. merrillii at 25% and 50% concentration was effective as anthelmintic on F. gigantica in vitro.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Henni Vanda
AU  - Reza Aulia Anwar
AU  - M. Daud AK
AU  - Farida Athaillah
AU  - Ummu Balqis
AU  - Siti Rani Ayuti
AU  - Frengki Frengki
PY  - 2021
DA  - 2021/05/03
TI  - Effect of Veitchia merrillii Extract on Mortality and Tegument Structure of Fasciola gigantica
BT  - Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020)
PB  - Atlantis Press
SP  - 65
EP  - 69
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210420.015
DO  - 10.2991/absr.k.210420.015
ID  - Vanda2021
ER  -