Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit
Guochang Ding, Weizhi Guo, Shaoning Ruan, Shubin Li, Yu Chen, Sizu Lin
Available Online September 2015.
- https://doi.org/10.2991/ifeesm-15.2015.133How to use a DOI?
- Cunninghamia lanceolata,Chinese fir ,water deficit, proteome.
- Chinese fir (Cunninghamia lanceolata) is an important commercial timber species in China. In the recent years, seasonal droughts occurred frequently in the fir growing areas and made a great impact on its production. In this study, PEG-6000 was used to simulate water deficit, while the drought-resistant FS43 and drought sensitive FS38 fir strains were selected as experimental materials. A two-dimensional electrophoresis technology was used for proteome analysis of leave protein comparisons between the two fir strains under water deficit. A total number of 24 differentially expressed protein spots were detected, of which five were identified by the tandem mass spectrometry and divided into four categories: (a) Functional enzymes involved in photosynthesis: the light-harvesting chlorophyll a/b-binding proteins, ribulose-1, 5-bisphosphate carboxylase/oxygenase; (b) Functional enzymes involved in osmotolerance: myo-inositol 1-phosphate synthase; (c) Functional enzymes involved in carbohydrate metabolism: granule-bound starch synthase I; (d) A conifer specific antioxidant stress-related functional enzyme. Under water deficit, these enzymes with obvious differences in protein expression levels might be involved in the regulation of fir responses to seasonal drought stress.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Guochang Ding AU - Weizhi Guo AU - Shaoning Ruan AU - Shubin Li AU - Yu Chen AU - Sizu Lin PY - 2015/09 DA - 2015/09 TI - Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit BT - Proceedings of the 2015 International Forum on Energy, Environment Science and Materials PB - Atlantis Press SP - 714 EP - 723 SN - 2352-5401 UR - https://doi.org/10.2991/ifeesm-15.2015.133 DO - https://doi.org/10.2991/ifeesm-15.2015.133 ID - Ding2015/09 ER -