Design of a weak fluorescence detection circuit in molecular diagnosis

DOI

10.2991/iiicec-15.2015.413How to use a DOI?

Keywords

Molecular diagnosis; weak fluorescence detection; photomultiplier tubes

Abstract

Fluorescence is generally adopted in molecular diagnosis. In order to detect weak fluorescence signal, a weak fluorescence detection circuit has been design based on a photomultiplier tube. The circuit mainly consists of three parts which are a photoelectric converter circuit, a pre-amplifier circuit and a filter circuit. This circuit is able to receive weak fluorescent signals and convert them into electrical signals effectively. As a result, important information such as the concentration of corresponding reagnet can be obtained by analyzing the intensity of fluorescence received. It is experimentally demonstrated that the designed circuit can be used in weak fluorescence detecting efficiently. Its detection limitation is as low as the fluorescent signal emitted from quantum dots of 10-10mol/ml, making it suitable for low fluorescence detection in molecular diagnosis.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Huajuan Bai
AU  - Jihong Feng
AU  - Hong Zhang
PY  - 2015/03
DA  - 2015/03
TI  - Design of a weak fluorescence detection circuit in molecular diagnosis
BT  - Proceedings of the 2015 International Industrial Informatics and Computer Engineering Conference
PB  - Atlantis Press
SP  - 1899
EP  - 1902
SN  - 2352-538X
UR  - https://doi.org/10.2991/iiicec-15.2015.413
DO  - 10.2991/iiicec-15.2015.413
ID  - Bai2015/03
ER  -