Functional evaluation of bacterial surface displayed P domain from human norovirus capsid proteins
Shaofeng Rong, Yue Zhou, Mengya Niu, Qianqian Li, Ming Wang, Shimin Guan, Shuo Zhang, Baoguo Cai
Available Online November 2016.
- https://doi.org/10.2991/imst-16.2016.41How to use a DOI?
- human norovirus; P domain; cell surface display; receptor binding properties.
- Human noroviruses (HuNoVs) are the major cause to acute nonbacterial gastroenteritis outbreaks. The receptors of HuNoVs are difficult to identify because HuNoVs cannot be easily cultivated in vitro and they lack effective animal models. To develop an effective method for mining functional viral receptors, engineering Escherichia coli were constructed to display P domain of capsid protein of HuNoVs (GI.1 or GII.4) on the cell surface, using the N-terminal of the ice nucleation protein (InaQN). In this study, membrane protein-porcine gastric mucin binding ELISA methods were employed to confirm the surface display efficiency. The receptor binding capacity of InaQN-P (GI.1 or GII.4) fusion proteins on the bacterial surface was evaluated through whole-cell fluorescence immunoassay with saliva-based histo-blood group antigens. Results revealed remarkable characteristic of antigenicity and receptor binding properties. Therefore, this bacterial surface display system should be considered as a candidate to mine and investigate HuNoV receptors in vivo or in vitro.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Shaofeng Rong AU - Yue Zhou AU - Mengya Niu AU - Qianqian Li AU - Ming Wang AU - Shimin Guan AU - Shuo Zhang AU - Baoguo Cai PY - 2016/11 DA - 2016/11 TI - Functional evaluation of bacterial surface displayed P domain from human norovirus capsid proteins BT - 2016 International Conference on Innovative Material Science and Technology (IMST 2016) PB - Atlantis Press SP - 277 EP - 284 SN - 1951-6851 UR - https://doi.org/10.2991/imst-16.2016.41 DO - https://doi.org/10.2991/imst-16.2016.41 ID - Rong2016/11 ER -