Pharmacokinetic of Flunixin Meglumine in Broiler Chicks by Enzyme-linked Immunosorbent Assay

DOI

10.2991/ahsr.k.211012.025How to use a DOI?

Keywords

Flunixin meglumine, Pharmacokinetic, ELISA, Chicks

Abstract

Objective: The aim of the study was to investigate the concentration of flunixin in the plasma of chicks to determine the pharmacokinetic parameters. Methods: A randomized controlled study was performed on forty five clinically healthy ross chicks of seven days old, chicks were administered into the peritoneum with a single dose of flunixin 18mg /kg b.wt, following injection of the drug, blood samples were collected at 0, 0.08, 0.15, 0.5, 1, 2, 4, 8, 24 h (five chicks per time) from the jugular vein after decapitation. Then the plasma was obtained, the concentrations of flunixin in plasma samples were determined utilizing Enzyme-Linked Immunosorbent Assay, the pharmacokinetic parameters were calculated by the PKSolver program. Time vs. concentration curve for flunixin was obtained from the program. The pharmacokinetic parameters were determined with non-compartmental models. Results: The concentration of flunixin in the plasma of chicks treated with flunixin by injection into the peritoneum at a dose (18 mg/kg) were 111.64 ± 1.8, 147.05 ± 1.4, 154.23 ± 1.3,141,12 ± 1.0, 136.70 ±1.2, 134.11 ±0.6, 125.76 ±1.9, 87, and 76 ±1.5 μg/ml at times 0.08, 0.25, 0.5, 1, 2, 4, 8 and 24 hours respectively. The maximum plasma concentration was 154.23μg/ml during a period of 0.5 hour of treatment. The terminal elimination half-life was 31hours, the terminal phase elimination rate constant was 0.021 h-1, the mean residence time was 45 h, and the area under the curve from time 0 to infinity was 6733.27 μg.h/ml. Conclusions: Based on the results of the study, we conclude the long elimination half-life of flunixin and the poor clearance from the animal body, which is reflected in the long effects of its analgesic, anti-inflammatory and antipyretic properties

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Zahraa Alhumdany
AU  - Yasser Albadrany
PY  - 2021
DA  - 2021/10/12
TI  - Pharmacokinetic of Flunixin Meglumine in Broiler Chicks by Enzyme-linked Immunosorbent Assay
BT  - Proceedings of the 1st International Ninevah Conference on Medical Sciences (INCMS 2021)
PB  - Atlantis Press
SP  - 149
EP  - 154
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211012.025
DO  - 10.2991/ahsr.k.211012.025
ID  - Alhumdany2021
ER  -