Auxin and Cytokinin Effect on In vitro Callus Induction of Maize (Zea mays L.) Srikandi Putih
- DOI
- 10.2991/absr.k.220401.001How to use a DOI?
- Keywords
- Maize srikandi Putih; 2,4-Dichlorophenoxyacetic acid (2,4-D); kinetin; in vitro
- Abstract
Maize plant (Zea mays L.) can be an alternative for sustainable forage because easy to obtain and can be cultivated in the dry season. Increasing plant biomass is needed to maximize maize plants as a forage crop through genetic mutations. Callus production by tissue culture is one of the planting materials used on plant mutagens. This study was done to identify the response of auxin and cytokinin hormone in callus induction of maize plant Srikandi Putih on root and stem explants. Kinetin and BAP (0.1 mg/L), and without cytokinin were combined with auxin 2,4-D (2,4-Dichlorophenoxyacetic acid) hormone, at two concentrations 1 and 2 mg/L. Stem explants from in vitro seedlings could produce callus 40-100%, while root explants from in vitro seedlings could produce callus 44-100%. MS medium containing 2,4-D 2 mg/L without cytokinin produced the highest callus both in roots and stems. The highest callus fresh weight (0.553g) was obtained in MS medium supplemented with 2,4-D 1 mg/L without cytokinin in the stem, while in the roots, 2,4-D 1 mg/L and Kinetin 0.1 mg/L produced the highest fresh weight (0085g).
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Muji Astutik AU - Bambang Suhartanto AU - Nafiatul Umami AU - Nilo Suseno AU - Miftahush Shirotul Haq PY - 2022 DA - 2022/04/11 TI - Auxin and Cytokinin Effect on In vitro Callus Induction of Maize (Zea mays L.) Srikandi Putih BT - Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021) PB - Atlantis Press SP - 1 EP - 5 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220401.001 DO - 10.2991/absr.k.220401.001 ID - Astutik2022 ER -