Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)

Auxin and Cytokinin Effect on In vitro Callus Induction of Maize (Zea mays L.) Srikandi Putih

Authors
Muji Astutik1, Bambang Suhartanto1, *, Nafiatul Umami1, Nilo Suseno1, Miftahush Shirotul Haq1
1Laboratory of Forage and Pasture Science, Department of Animal Nutrition and Feed Science, Faculty of Animal Science, University of Gadjah Mada, 55281 Yogyakarta, Indonesia.
*Corresponding author. Email: bamsuhar@ugm.ac.id
Corresponding Author
Bambang Suhartanto
Available Online 11 April 2022.
DOI
10.2991/absr.k.220401.001How to use a DOI?
Keywords
Maize srikandi Putih; 2,4-Dichlorophenoxyacetic acid (2,4-D); kinetin; in vitro
Abstract

Maize plant (Zea mays L.) can be an alternative for sustainable forage because easy to obtain and can be cultivated in the dry season. Increasing plant biomass is needed to maximize maize plants as a forage crop through genetic mutations. Callus production by tissue culture is one of the planting materials used on plant mutagens. This study was done to identify the response of auxin and cytokinin hormone in callus induction of maize plant Srikandi Putih on root and stem explants. Kinetin and BAP (0.1 mg/L), and without cytokinin were combined with auxin 2,4-D (2,4-Dichlorophenoxyacetic acid) hormone, at two concentrations 1 and 2 mg/L. Stem explants from in vitro seedlings could produce callus 40-100%, while root explants from in vitro seedlings could produce callus 44-100%. MS medium containing 2,4-D 2 mg/L without cytokinin produced the highest callus both in roots and stems. The highest callus fresh weight (0.553g) was obtained in MS medium supplemented with 2,4-D 1 mg/L without cytokinin in the stem, while in the roots, 2,4-D 1 mg/L and Kinetin 0.1 mg/L produced the highest fresh weight (0085g).

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
Series
Advances in Biological Sciences Research
Publication Date
11 April 2022
ISBN
978-94-6239-562-6
ISSN
2468-5747
DOI
10.2991/absr.k.220401.001How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

TY  - CONF
AU  - Muji Astutik
AU  - Bambang Suhartanto
AU  - Nafiatul Umami
AU  - Nilo Suseno
AU  - Miftahush Shirotul Haq
PY  - 2022
DA  - 2022/04/11
TI  - Auxin and Cytokinin Effect on In vitro Callus Induction of Maize (Zea mays L.) Srikandi Putih
BT  - Proceedings of the 6th International Seminar of Animal Nutrition and Feed Science (ISANFS 2021)
PB  - Atlantis Press
SP  - 1
EP  - 5
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220401.001
DO  - 10.2991/absr.k.220401.001
ID  - Astutik2022
ER  -