Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)

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Description of BHK-21Persistent Infected Cell Inoculated with Infectious Bovine Rhinotracheitis Virus

Authors
Dewi Hidayati, Isnoer Aini, Diah Pancawidyana, S Indarjulianto
Corresponding Author
Dewi Hidayati
Available Online December 2019.
DOI
10.2991/isessah-19.2019.12How to use a DOI?
Keywords
IBR, subtype 1.1, subtype 1.2b, UL36 gene, MDBK, BHK-21
Abstract

Infectious Bovine Rhinotracheitis (IBR) disease caused by Bovine Herpesvirus-1 (BHV-1), this diseases include in strategic diseases. Based on the genotype, this virus divided into several subtypes, including subtype 1.1; subtype 1.2a and subtype 1.2b. The previous genotypic study revealed that based on the insertion-deletion of UL36 gene, samples divided into subtype 1.1 and subtype 1.2. This study aim was comparing phenotypic images of isolates from Lampung and Bukittinggi in MDBK and BHK-21 cells. Samples of swab nasal and tracheal organ obtained from Balai Veteriner Lampung and Balai Veteriner Bukittinggi. The samples were cultured on MDBK and BHK-21 monolayer cell of 2 days old obtained from Pusvetma. The Cytopathogenic Effects (CPE) in MDBK and BHK-21 observed on day 2. In the MDBK cell, the CPE found as the grape-like cluster and apoptosis. The CPE of BHK-21 cell line showed syncytium and indicated persistent infection. At day four on BHK-21 cell, it observed the particular infected cell that showed differences in cell shape between samples that have proximity to subtype 1.1 and subtype 1.2. The infected cell of subtype 1.1 group, appear as elongated shape and linear pattern near the center area. Inversely, the samples grouped as subtype 1.2 appear as rounded cell and circular pattern of the center area

Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
Series
Advances in Health Sciences Research
Publication Date
December 2019
ISBN
978-94-6252-869-7
ISSN
2468-5739
DOI
10.2991/isessah-19.2019.12How to use a DOI?
Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Dewi Hidayati
AU  - Isnoer Aini
AU  - Diah Pancawidyana
AU  - S Indarjulianto
PY  - 2019/12
DA  - 2019/12
TI  - Description of BHK-21Persistent Infected Cell Inoculated with Infectious  Bovine Rhinotracheitis Virus
BT  - Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
PB  - Atlantis Press
SP  - 94
EP  - 98
SN  - 2468-5739
UR  - https://doi.org/10.2991/isessah-19.2019.12
DO  - 10.2991/isessah-19.2019.12
ID  - Hidayati2019/12
ER  -