Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)

BHK -21 Cell Grown on Microcarrier System Increasing The Capacity of Rabies Vaccine

Authors
Diah Pancawidyana, Wriningati Wriningati, Evy Setyorinie, Kiki Restika, Agung Suganda
Corresponding Author
Diah Pancawidyana
Available Online December 2019.
DOI
10.2991/isessah-19.2019.14How to use a DOI?
Keywords
BHK-21 cell, microcarrier, rabies virus, vaccine
Abstract

Rabies is a fatal zoonotic disease caused by the rabies virus. Vaccination is the most effective way to prevent and control rabies. Pusvetma as one of national rabies vaccine producer needs to increase the production capacity to meet the market demand of this vaccine. The use of microcarrier for propagating the Baby Hamster Kidney (BHK)-21 is expected to increase production capacity of Pusvetma’s rabies vaccine. This study aims to compare the efficacy of two propagation methods of BHK-21 cell: 1) microcarrier system using bioreactor and 2) conventional method using roller bottle. Microcarrier cell culture using 2 × 10⁸ cells in bioreactor contain 850 carriers, whilst conventional method using 26 roller bottles contain 5-10 × 106 cells/bottle. We propagated 65 ml of Rabies virus Pasteur strain into microcarrier cell culture and 5 ml same virus into roller bottle (virus titer 107 LD50/ml). Our result showed that BHK-21 cell growth was 12.96 × 108 cells with 500 ml Eagle Medium, virus titer 106,7 LD50/ml in bioreactor, and 13 × 108 cells with 5200 ml Eagle medium, virus titer obtained was 106,0 LD50/ml in conventional method . The concluded was the usage of microcarrier more effective and efficient than conventional method.

Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
Series
Advances in Health Sciences Research
Publication Date
December 2019
ISBN
978-94-6252-869-7
ISSN
2468-5739
DOI
10.2991/isessah-19.2019.14How to use a DOI?
Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Diah Pancawidyana
AU  - Wriningati Wriningati
AU  - Evy Setyorinie
AU  - Kiki Restika
AU  - Agung Suganda
PY  - 2019/12
DA  - 2019/12
TI  - BHK -21 Cell Grown on Microcarrier System Increasing  The Capacity  of Rabies Vaccine
BT  - Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
PB  - Atlantis Press
SP  - 86
EP  - 89
SN  - 2468-5739
UR  - https://doi.org/10.2991/isessah-19.2019.14
DO  - 10.2991/isessah-19.2019.14
ID  - Pancawidyana2019/12
ER  -