Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)

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Epitope Prediction from Genes Encoding F Protein of Newcastle Disease Virus (NDV) Isolates Swan (Cygnus Olor) for Vaccine Development to Prevent Infectious Disease

Authors
Naimah Putri, Rahaju Ernawati, Suwarno, Jola Rahmahani, Fedik Rantam
Corresponding Author
Naimah Putri
Available Online December 2019.
DOI
10.2991/isessah-19.2019.26How to use a DOI?
Keywords
Epitope, F Protein, Homology, Newcastle disease virus, Phylogenetic tree, RT-PCR
Abstract

Currently, the poultry industry is threatened by virulent viruses of endemic disease that can cause major economis losses to this sector. Aim of this research was conducted to epitope prediction from genes encoding F protein of Newcastle Disease Virus (NDV) isolated from waterfowl. Samples of this research collected from cloacal swabs of seventy one waterfowls. Consisting of twenty four domestic swan, thirty three Muscovy ducks, and fourteen domestic ducks form waterfowls in East Java Province, and also positive LaSota Isolates (ATCC). Samples were isolated in embryonated chicken eggs (ECEs) with Specific Pathogen Free (SPF) and identified by HA test and confirmed as NDV by HI test. Positive samples were performed by RT-PCR using forward and reverse primer with target of 699 bp. The sequencing results were analyzed by forward and reverse primer to generate the complete gene. Candidate epitopes were analysed by predictions tools from Immune Epitope Database online program. One epitope was predicted as a peptide vaccine for B cell (HGKILDPGWRPPGARWAPDLLP) from ND/AG3/2018 sample and more likely to be immunogen vaccine candidate based on epitope prediction immunogenic epitope candidates have hisghest log score

Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
Series
Advances in Health Sciences Research
Publication Date
December 2019
ISBN
978-94-6252-869-7
ISSN
2468-5739
DOI
10.2991/isessah-19.2019.26How to use a DOI?
Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Naimah Putri
AU  - Rahaju Ernawati
AU  - Suwarno
AU  - Jola Rahmahani
AU  - Fedik Rantam
PY  - 2019/12
DA  - 2019/12
TI  - Epitope Prediction from Genes Encoding F Protein of Newcastle Disease Virus (NDV) Isolates Swan (Cygnus Olor) for Vaccine Development to Prevent Infectious Disease
BT  - Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
PB  - Atlantis Press
SP  - 40
EP  - 43
SN  - 2468-5739
UR  - https://doi.org/10.2991/isessah-19.2019.26
DO  - 10.2991/isessah-19.2019.26
ID  - Putri2019/12
ER  -