Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019)

Application of omix technologies in studying of BLV biological diversity by gag gene

Authors
Natalia Blazhko, Sultan Vyshegurov, Kirill Shatokhin
Corresponding Author
Kirill Shatokhin
Available Online June 2019.
DOI
10.2991/ispc-19.2019.5How to use a DOI?
Keywords
cattle, BLV, leukemia, gag gene, PCR analysis, omix technologies
Abstract

This paper is the part of a large-scale project concerning studying of bovine leukemia virus biological diversity and of the influence of BLV structural characteristics on virulence and hematolytic blood parameters of infected animals in Western Siberia. This part is devoted to the study of the influence of haplotypes which are determined by mutations in gag gene on several blood parameters and on the number of viral particles per 1000 healthy cells. Emphasis is made on the importance of bioinformatics analysis for processing large data arrays during the multivariant analysis of BLV carriers’ parameters. It was established that about ¾ of infected animals were infected with II haplotype virus, whereas the remaining part was infected with BLV of I haplotype. The number of leukocytes, including lymphocytes, erythrocytes, and platelets in the blood of animals infected with II haplotype virus, was generally higher than that of the carriers of I haplotype. Viral load in carriers of II haplotype was 800-1000 viral particles per 1000 healthy cells, whereas the number of viral particles per 1000 healthy cells in the blood of individuals with I haplotype ranged from 600 to 900. It is worth noting that among carriers of I haplotype there were no animals with clinical signs of leukemia, and viral load in healthy animals exceeded that in suspicious individuals. In general, it was shown that gag gene, along with LTR region, is a rather significant factor in maintaining of “viral load – immune response” correlation.

Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019)
Series
Advances in Intelligent Systems Research
Publication Date
June 2019
ISBN
10.2991/ispc-19.2019.5
ISSN
1951-6851
DOI
10.2991/ispc-19.2019.5How to use a DOI?
Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Natalia Blazhko
AU  - Sultan Vyshegurov
AU  - Kirill Shatokhin
PY  - 2019/06
DA  - 2019/06
TI  - Application of omix technologies in studying of BLV biological diversity by gag gene
BT  - Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019)
PB  - Atlantis Press
SP  - 18
EP  - 21
SN  - 1951-6851
UR  - https://doi.org/10.2991/ispc-19.2019.5
DO  - 10.2991/ispc-19.2019.5
ID  - Blazhko2019/06
ER  -