Influence of Anti-tuberculosis Drug KIM-M2 on Morphology of Lymph Nodes, Spleen, Liver and Lungs of Guinea Pigs Infected with M. bovis
- DOI
- 10.2991/ispc-19.2019.41How to use a DOI?
- Keywords
- cattle, anti-tuberculosis drug KIM-M2, histomorphology, organs
- Abstract
Conjugation of immunogenic fraction separated from microbial cells of BCG strain with stimulating component proved to be a promising direction in the creation of anti-tuberculosis drugs. One of such components is KIM-M2, a complex of thermostable soluble antigens extracted from microbial cells of BCG strain incubated with formalin and conjugated with polyvinyl pyrrolidone and polyethyleneglycol. To study the impact of KIM-M2 on the morphology of lymph nodes, spleen, lungs and liver of guinea pigs, the animals of group 1 (control, n = 5) were injected subcutaneously with a virulent culture of M. bovis strain 8 at a dose of 0.001 mg/ml. Animals of group 2 (n = 5 subjects) were injected subcutaneously with KIM-M2 at a dose of 0.5 mg/ml of protein, then 30 days later they were injected with a virulent culture of M. bovis strain 8, subcutaneously at a dose of 0.001 mg/ml. Animals of group 3 (n = 5 subjects) were injected subcutaneously with a virulent culture of M. bovis strain 8 at a dose of 0.001 mg/ml, 14 days later they were injected with KIM-M2 at a dose of 0.5 mg/ml of protein. Histomorphologic changes in animals of group 1 can be considered as morphological equivalent of immune suppression reaction. At the same time, the animals from group 2 and especially from experimental group 3 showed an increase of immunogenesis that can be proved by a profound proliferation of lymphocytes and macrophages.
- Copyright
- © 2019, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Vasiliy Vlasenko AU - Valentina Pleshakova AU - Yuriy Gichev PY - 2019/06 DA - 2019/06 TI - Influence of Anti-tuberculosis Drug KIM-M2 on Morphology of Lymph Nodes, Spleen, Liver and Lungs of Guinea Pigs Infected with M. bovis BT - Proceedings of the International Scientific and Practical Conference “Digital agriculture - development strategy” (ISPC 2019) PB - Atlantis Press SP - 183 EP - 187 SN - 1951-6851 UR - https://doi.org/10.2991/ispc-19.2019.41 DO - 10.2991/ispc-19.2019.41 ID - Vlasenko2019/06 ER -