Exploration of the Potential of Dandang Gendis Leaf Extract (Clinacanthus nutans L) as an In Vitro Antidiabetic (A-Glucosidase Enzyme Inhibition Test) and In Vivo (Decrease Glucose Levels and Glut-2 Expression in Mice)

Artati; Ahyar Ahmad; Abdul Karim; Sofa Fajriah; Harningsih Karim; Nuradi; Arfiani Nur; Ummi Zahra; Syahida Djasang; Muhammad Nasir; Siti Nurul Fajriah

doi:10.2991/978-94-6463-228-6_29

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Exploration of the Potential of Dandang Gendis Leaf Extract (Clinacanthus nutans L) as an In Vitro Antidiabetic (A-Glucosidase Enzyme Inhibition Test) and In Vivo (Decrease Glucose Levels and Glut-2 Expression in Mice)

Authors

Artati¹^{, 2}, Ahyar Ahmad¹^{, *}, Abdul Karim¹, Sofa Fajriah³, Harningsih Karim⁴, Nuradi², Arfiani Nur⁵, Ummi Zahra⁵, Syahida Djasang², Muhammad Nasir², Siti Nurul Fajriah²

¹Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar, 90245, Indonesia

²Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 District. Rappocini Kel. Banta-Bantaeng, Makassar, 90222, Indonesia

³Chemical Research Center, Indonesian Institute of Sciences, Puspiptek Serpong, South Tangerang, Banten, 15314, Indonesia

⁴Department of Pharmacy, Yamasi School of Pharmacy, Makassar, Indonesia

⁵Department of Chemistry, Faculty of Science and Technology, Alauddin State Islamic University Makassar, Jalan HM Yasin Limpo, Gowa, Makassar, 92113, Indonesia

^*Corresponding author. Email: ahyarahmad@gmail.com

Corresponding Author

Ahyar Ahmad

Available Online 22 August 2023.

DOI: 10.2991/978-94-6463-228-6_29 How to use a DOI?
Keywords: Antidiabetic; α-Glucosidase; Dandang Gendis Leaves
Abstract: Diabetes mellitus is thought to play a role in increasing free radicals, de-creasing antioxidants and triggering oxidative stress, and causing damage to GLUT 2. GLUT 2 on the pancreatic cell membrane is responsible for the transport of blood sugar in mice which will stimulate insulin secretion. Dandang gendis (Clinacanthus nutans L) leaves havethe potential as antidiabetic because of the flavonoid compounds that function as antioxidants. Antioxidants are compounds that can reduce the negative impact of oxidants in inhibiting. Dandang gendis leaf extract (DGLE) was obtained by maceration extracting dandang gendis leaves. Methods. In vitro α-glucosidase enzyme inhibition test and in vivo test of blood sugar (glucose) levels and Glut-2 expression in mice. Research result in the α-glucosidase enzyme inhibition test gave an IC50 value of 7.27 ppm and was active as an antidiabetic. There was a difference in the effect of giving on reducing blood sugar levels in mice, with the optimal concentration of DGLE 100 mg/kgBW which has activity in reducing blood sugar levels in mice by 51.60%. There was an increase in Glut-2 expression on DGLE distribution. Conclusion DGLE has the potential as an antidiabetic with the mechanism of inhibition of the α-glucosidase enzyme, decreasing blood sugar levels and increasing Glut-2 expression.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 4th International Seminar on Science and Technology (ISST 2022)
Series: Advances in Physics Research
Publication Date: 22 August 2023
ISBN: 10.2991/978-94-6463-228-6_29
ISSN: 2352-541X
DOI: 10.2991/978-94-6463-228-6_29 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Artati
AU  - Ahyar Ahmad
AU  - Abdul Karim
AU  - Sofa Fajriah
AU  - Harningsih Karim
AU  - Nuradi
AU  - Arfiani Nur
AU  - Ummi Zahra
AU  - Syahida Djasang
AU  - Muhammad Nasir
AU  - Siti Nurul Fajriah
PY  - 2023
DA  - 2023/08/22
TI  - Exploration of the Potential of Dandang Gendis Leaf Extract (Clinacanthus nutans L) as an In Vitro Antidiabetic (A-Glucosidase Enzyme Inhibition Test) and In Vivo (Decrease Glucose Levels and Glut-2 Expression in Mice)
BT  - Proceedings of the 4th International Seminar on Science and Technology (ISST 2022)
PB  - Atlantis Press
SP  - 251
EP  - 261
SN  - 2352-541X
UR  - https://doi.org/10.2991/978-94-6463-228-6_29
DO  - 10.2991/978-94-6463-228-6_29
ID  - 2023
ER  -

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