Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)

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Molecular Verification of Sex-separated Straw of Simmental Cattle (Bos taurus) by Polymerase Chain Reaction (PCR)

Authors
Aulia Rahman1, Asmarani Kusumawati2, *, Agung Budiyanto2, Yemi Ulviani1, Ilham Fathurrahman1, Kurniawan Dwi Prihantoko3, Lalu Unsunnidhal3
1Master of Biotechnology Programs, Graduate Schools, Universitas Gadjah Mada, Yogyakarta.
2Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2, Karangmalang, Sleman, Yogyakarta.
3Doctor of Veterinary Science Programs, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta.
*Corresponding author. Email:uma_vet@ugm.ac.id
Corresponding Author
Asmarani Kusumawati
Available Online 24 February 2022.
DOI
10.2991/absr.k.220207.046How to use a DOI?
Keywords
sex-separated straw; SRY gene; primer design; PCR; Simmental cattle
Abstract

The application of sexing technology to separate X and Y spermatozoa is a strategic step to fulfill the beef demand in Indonesia. Current sexing methods like gradient density percoll, bovine serum albumin, and spectrophotometry did not have 100% accuracy to separate between X and Y spermatozoa. The molecular verification process by Polymerase Chain Reaction (PCR) assay becomes an important step to verify the sex-separated straw of Simmental cattle, so a higher percentage of sex suitability of calves expected by farmers is achieved. This research aims to develop a new primer for Y-chromosome bearing spermatozoa in the sex-separated straw of Simmental cattle by PCR assay. Primer design for PCR assay is an important step. In this research, primer was designed by bioinformatics analysis using primer3plus based on Sex determining Region Y (SRY) gene, which encodes the male sex determination in spermatozoa. Genomic DNA of spermatozoa was isolated by PureLink Genomic DNA Mini Kit (Invitrogen, USA). Primer design by bioinformatics analysis obtained primer products of 232 bp length with 50% of GC content. The amplification of SRY primer produced a single fragment of 232 bp, respectively. Thus, this primer can be used to verify the copy of the SRY gene in the sex-separated straw of Simmental cattle.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
Series
Advances in Biological Sciences Research
Publication Date
24 February 2022
ISBN
10.2991/absr.k.220207.046
ISSN
2468-5747
DOI
10.2991/absr.k.220207.046How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Aulia Rahman
AU  - Asmarani Kusumawati
AU  - Agung Budiyanto
AU  - Yemi Ulviani
AU  - Ilham Fathurrahman
AU  - Kurniawan Dwi Prihantoko
AU  - Lalu Unsunnidhal
PY  - 2022
DA  - 2022/02/24
TI  - Molecular Verification of Sex-separated Straw of Simmental Cattle (Bos taurus) by Polymerase Chain Reaction (PCR)
BT  - Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021)
PB  - Atlantis Press
SP  - 223
EP  - 226
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220207.046
DO  - 10.2991/absr.k.220207.046
ID  - Rahman2022
ER  -