Proceedings of the 2015 International Conference on Industrial Technology and Management Science

Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins

Authors
Lielian Zuo, Meijuan Zhu, Shujuan Du, Qijia Yan, Haibo Yuan, Jing Yang, Guiyuan Li, Jianhong Lu, Liming He
Corresponding Author
Lielian Zuo
Available Online November 2015.
DOI
https://doi.org/10.2991/itms-15.2015.253How to use a DOI?
Keywords
Bimolecular complementation; Epstein-Barr virus nuclear antigen 1; Protein-protein interaction
Abstract
Epstein-Barr virus nuclear antigen 1 (EBNA1) is essential for EBV episome’s replication and mitotic segregation. During this process, EBNA1 may interact with a lot of host factors. Bimolecular complementation (BiMC) assay is a useful tool for a detection of protein-protein interaction in living cells. In the present study, a plasmid for expressing a fusion protein CYFP-EBNA1 was constructed. As expected, it could express CYFP-EBNA1 and localize in the cellular nuclear. The result also demonstrated that the plasmid is allowed to be used in the BiMC system.
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Proceedings
2015 International Conference on Industrial Technology and Management Science
Part of series
Advances in Computer Science Research
Publication Date
November 2015
ISBN
978-94-6252-123-0
ISSN
2352-538X
DOI
https://doi.org/10.2991/itms-15.2015.253How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Lielian Zuo
AU  - Meijuan Zhu
AU  - Shujuan Du
AU  - Qijia Yan
AU  - Haibo Yuan
AU  - Jing Yang
AU  - Guiyuan Li
AU  - Jianhong Lu
AU  - Liming He
PY  - 2015/11
DA  - 2015/11
TI  - Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins
BT  - 2015 International Conference on Industrial Technology and Management Science
PB  - Atlantis Press
SN  - 2352-538X
UR  - https://doi.org/10.2991/itms-15.2015.253
DO  - https://doi.org/10.2991/itms-15.2015.253
ID  - Zuo2015/11
ER  -