Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins
Lielian Zuo, Meijuan Zhu, Shujuan Du, Qijia Yan, Haibo Yuan, Jing Yang, Guiyuan Li, Jianhong Lu, Liming He
Available Online November 2015.
- https://doi.org/10.2991/itms-15.2015.253How to use a DOI?
- Bimolecular complementation; Epstein-Barr virus nuclear antigen 1; Protein-protein interaction
- Epstein-Barr virus nuclear antigen 1 (EBNA1) is essential for EBV episome’s replication and mitotic segregation. During this process, EBNA1 may interact with a lot of host factors. Bimolecular complementation (BiMC) assay is a useful tool for a detection of protein-protein interaction in living cells. In the present study, a plasmid for expressing a fusion protein CYFP-EBNA1 was constructed. As expected, it could express CYFP-EBNA1 and localize in the cellular nuclear. The result also demonstrated that the plasmid is allowed to be used in the BiMC system.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Lielian Zuo AU - Meijuan Zhu AU - Shujuan Du AU - Qijia Yan AU - Haibo Yuan AU - Jing Yang AU - Guiyuan Li AU - Jianhong Lu AU - Liming He PY - 2015/11 DA - 2015/11 TI - Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins PB - Atlantis Press SP - 1047 EP - 1050 SN - 2352-538X UR - https://doi.org/10.2991/itms-15.2015.253 DO - https://doi.org/10.2991/itms-15.2015.253 ID - Zuo2015/11 ER -