Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)
- DOI
- 10.2991/absr.k.210621.081How to use a DOI?
- Keywords
- Antibiotic resistance, identification, bacteria, wildlife
- Abstract
Antibiotic resistance is one of the most serious health issues in the world which is commonly attributed to the overuse of antibiotics whether in human or animal treatment. The dispersion of antibiotic resistance genes not only found in bacteria from the clinical sample but also found in wildlife animals. Therefore, the aim of this research is to isolate, identify and perform antibiotic resistance assay in bacterial isolates from fruit bat Chironax melanocephalus. Animal samples were taken by using a mist net installed in one of the spots in Gunung Halimun Salak National Park (TNGHS). Bacteria sample was taken using an oral and rectal swab. The bacteria were then isolated in the laboratory by using xylose lactose deoxycholate (XLD) agar medium. There were two bacteria successfully isolated and identified using 16s rDNA amplification. The identification was performed by comparing the 16s rDNA genes to the two online databases i.e BLAST-N in Genebank and 16s-Based ID in the EzBioCloud database. According to the identification results, isolate 4A.1 was identified as Proteus mirabilis while isolate 5.B.2 is identified as Serratia marcescens. Then antibiotic susceptibility test was performed using the disk diffusion method using four different antibiotics disk i.e oxacillin, cefoxitin, amikacin and amoxicillin-clavulanic acid. The resistance profile of each bacteria is different between P. mirabilis and S. marcescens in each antibiotic. However, all isolates were found susceptible against amikacin 30 µg.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Masrukhin AU - Sugiyono Saputra AU - Syaiful Rizal AU - Pangda Sopha Sushadi AU - Nanang Supriatna PY - 2021 DA - 2021/06/23 TI - Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus) BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 478 EP - 484 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.081 DO - 10.2991/absr.k.210621.081 ID - 2021 ER -