Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Cloning and Expression of Recombinant Protein MPT63 of Mycobacterium tuberculosis Indonesian Isolate as Serodiagnostic Latent Tuberculosis

Authors
Rosana Agus, Ian Imanuel Fidhatami, Mochammad Hatta
Corresponding Author
Rosana Agus
Available Online 23 June 2021.
DOI
10.2991/absr.k.210621.065How to use a DOI?
Keywords
MPT63, Latent, Serodiagnostic, Recombinant
Abstract

Indonesia and 13 other countries are included in High Burden Countries (HBC) in Tuberculosis (TB) cases. One of the problems in TB control in Indonesia is detecting latent TB appropriately. Diagnosis of latent TB does not have a gold standard. Some tests to detect latent TB have limitations in TB endemic areas such as Indonesia. Therefore, we need a fast, effective, and accurate method to diagnose TB. One of the potential proteins is MPT63, which is coded by the Rv 1926c gene. It is known that MPT 63 can induce Th1 cell reactivity and proliferation of IFN-λ. The purpose of this research was to clone and produce the recombinant protein MPT 63 from Mycobacterium tuberculosis. The method is Rv 1926c ligation to the pQE30-Xa expression vector and transformation to Escherichia coli BL21 host cell. Recombinant protein production was carried out by growing recombinant clones Rv1926-pQE on Luria Bertani medium by IPTG induction. The transformation results obtained white colonies and blue colonies. The growth of Escherichia coli BL21 carrying a recombinant plasmid is characterized by a change in the color of the medium. MPT63 recombinant protein was characterized by SDS-PAGE with a size of 16 kDa.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
Series
Advances in Biological Sciences Research
Publication Date
23 June 2021
ISBN
10.2991/absr.k.210621.065
ISSN
2468-5747
DOI
10.2991/absr.k.210621.065How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Rosana Agus
AU  - Ian Imanuel Fidhatami
AU  - Mochammad Hatta
PY  - 2021
DA  - 2021/06/23
TI  - Cloning and Expression of Recombinant Protein MPT63 of Mycobacterium tuberculosis Indonesian Isolate as Serodiagnostic Latent Tuberculosis
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 387
EP  - 390
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.065
DO  - 10.2991/absr.k.210621.065
ID  - Agus2021
ER  -