Antibacterial Activities of Staphylococcus sciuri APD7 Isolate Sponge-Associated Aplysina sp. Against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus

DOI

10.2991/absr.k.210621.059How to use a DOI?

Keywords

Aplysina sp, Enggano island, Antibacterial activity, Identification of 16S rRNA gene

Abstract

Sponges including multicellular animals, do not have actual tissues and organs, and are the simplest animals structurally. Sponges are also a group of marine life that is symbiotic with the microbial community. Microbes that have formed a symbiotic relationship with a sponge will produce secondary metabolites which also protect their host from pathogens. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp sponge is known to contain metabolites with antimicrobial, repellent properties, fighting predators, cytotoxic activity of breast cancer cells, has an antibiotic and anti-inflammatory effect on gram-positive bacteria. This study aims to identify of potential isolates associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolates were screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD7 isolate could inhibit Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in vitro. APD7 isolate was identified molecularly using of 16S rRNA genes analysis and it genetically close with Staphylococcus sciuri.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Delfiani Anggias Putri
AU  - Risky Hadi Wibowo
AU  - Sipriyadi
AU  - Welly Darwis
AU  - Choirul Muslim
AU  - Salprima Yudha
AU  - Hizkia Puspa Pertiwi
PY  - 2021
DA  - 2021/06/23
TI  - Antibacterial Activities of Staphylococcus sciuri APD7 Isolate Sponge-Associated Aplysina sp. Against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 346
EP  - 352
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.059
DO  - 10.2991/absr.k.210621.059
ID  - Putri2021
ER  -