Antimicrobial Activity of Pseudomonas fluorescens and Bacillus subtilis on Different Dilution Concentrations Against Various Citrus Post-Harvest Pathogens
- DOI
- 10.2991/absr.k.210621.089How to use a DOI?
- Keywords
- Antimicrobial, Bacillus subtilis, dilution, post-harvest, Pseudomonas flourescens
- Abstract
Pseudomonas flourescens and Bacillus subtilis are common antagonistic bacteria to various pathogens. These bacteria can produce various antimicrobial substances such as chitinase, siderophore, and antibiotic. This research aims to obtain the optimum dilution concentrations of Pseudomonas flourescens and Bacillus subtilis that can effectively inhibit the growth of Penicillium digitatum, P. paradoxum, and Aspergillus sp. This research was carried out in a complete randomized design with five different dilution concentrations of Pseudomonas flourescens and Bacillus subtilis ranging from 10-1 to 10-5. The antimicrobial activity tests against fungal pathogens were carried out in triplicate using a dual culture method. P. flourescens and B. subtilis displayed strong antimicrobial activities against all target pathogens. The highest inhibition activity of P. flourescens and B. subtilis was recorded against Aspergillus sp. with 45.7 % and 35.92 % and cell density of 168 x 106 CFU/mL and 14 x 106 CFU/mL, respectively. The highest antimicrobial activity was recorded at the 10-1 and 10-4 for P. flourescens and B. subtilis, respectively. The antimicrobial activity of P.flourescens and B.subtilis against P. digitatum and P. paradoxum are ranging from 17.59 % to 27.73 %.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Unun Triasih AU - Yogi Adhi Nugroho AU - Sri Widyaningsih PY - 2021 DA - 2021/06/23 TI - Antimicrobial Activity of Pseudomonas fluorescens and Bacillus subtilis on Different Dilution Concentrations Against Various Citrus Post-Harvest Pathogens BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 535 EP - 539 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.089 DO - 10.2991/absr.k.210621.089 ID - Triasih2021 ER -