Proceedings of the 2016 6th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2016)

Preparation of Carica Papaya Lipase and its Catalytic Resolution on Flurbiprofen Methyl Ester

Authors
Chunyu Li, Huiying Lin, Wei Zhang, Jiaying Xin
Corresponding Author
Chunyu Li
Available Online December 2016.
DOI
https://doi.org/10.2991/mcei-16.2016.99How to use a DOI?
Keywords
Carica papaya lipase; Flubiprofen; Chiral separation
Abstract
Carica papaya lipase(CPL) storeed in the crude papain was explored as enantioselective biocatalys for the hydrolysis resolution of flubiprofen methyl ester. According to its natural properties,13 percent of carica papaya lipase was obtained from crude enzyme extraction where the activity of the lipase was 90U/g. The optimum hydrolysis conditions of carica papaya lipase were as below, the lyophilization time was 8 hours, the reaction temperature was 35 , the pH was 7.0. Based on the hydrolysis catalyzed by carica papaya lipase, it was found that carica papaya lipase has a good tolerance to the low polar organic solvents. On the basis of single factor experiments the optimal conditions was built when the reaction was carried out in the iso-octane with 10mg/mL of flurbiprofen methyl ester, 70mg of carica papaya lipase, 5% of the buffer, 150rpm of the stirring speed, at 35 for 7 days. The maximum conversion was 14%, and the enantiomeric excess of product was 87%. Comparison of two methods for flubiprofen resolution indicated that the second resolution method can get a higher optical purity of flurbiprofen than that of the single lipase resolution method.
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Proceedings
2016 6th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2016)
Part of series
Advances in Intelligent Systems Research
Publication Date
December 2016
ISBN
978-94-6252-282-4
ISSN
1951-6851
DOI
https://doi.org/10.2991/mcei-16.2016.99How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Chunyu Li
AU  - Huiying Lin
AU  - Wei Zhang
AU  - Jiaying Xin
PY  - 2016/12
DA  - 2016/12
TI  - Preparation of Carica Papaya Lipase and its Catalytic Resolution on Flurbiprofen Methyl Ester
BT  - 2016 6th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2016)
PB  - Atlantis Press
SP  - 480
EP  - 483
SN  - 1951-6851
UR  - https://doi.org/10.2991/mcei-16.2016.99
DO  - https://doi.org/10.2991/mcei-16.2016.99
ID  - Li2016/12
ER  -