Fabrication of Tyrosinase Nano-biosensor for Catechol Inspection

DOI

10.2991/mebe-15.2015.40How to use a DOI?

Keywords

Encapsulation; Tyrosinase; Biosensor; Phenolic compounds; Liposome

Abstract

A novel tyrosinase (Tyr) biosensor based on liposome bioreactor and chitosan (CS) nano-composite has been developed for the detection of phenolic compounds. The encapsulation efficiency and drug loading content of the Tyr-loaded Liposome-based bioreactors were about 46.35± 0.85% and 41.15±0.95%, respectively. The glassy carbon electrode (GCE) was alternately immersed in CS and Tyr liposome bioreactor (TLB) to assemble bilayer films [(CS/TLB)/GCE]. The presence of Tyr in the biosensor confirmed by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical measurements. The results indicated that the biosensor was applied to detect catechol with a broad linear range from 0.25 nM to 25 M, the detection limit was brought down to 0.125 nM. The novel biosensor exhibits good repeatability and stability.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Huanan Guan
AU  - Benjun Li
PY  - 2015/04
DA  - 2015/04
TI  - Fabrication of Tyrosinase Nano-biosensor for Catechol Inspection
BT  - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
PB  - Atlantis Press
SP  - 167
EP  - 170
SN  - 2352-5401
UR  - https://doi.org/10.2991/mebe-15.2015.40
DO  - 10.2991/mebe-15.2015.40
ID  - Guan2015/04
ER  -