Study on Distribution Behavior in Aqueous Two-phase System about Four Kinds of Alkaloids in Coptis Chinensis

DOI

10.2991/mebe-15.2015.104How to use a DOI?

Keywords

coptis chinensis alkaloids; aqueous two-phase extraction; ultraviolet spectrophotometry.

Abstract

Using aqueous two-phase to extract alkaloids in coptis chinensis. By changing the aqueous two-phase systems (molecular weight and concentration of polyethylene glycol (PEG), kinds and concentration of salt), and researched the distribution behavior of four major alkaloids in rhizoma coptidis: berberine, coptisine, palmatine and jatrorrhizin in aqueous two-phase system. Combined with ultraviolet Spectrophotometric to detect the extraction. Found that the four alkaloids extraction from high to low as follows: berberine, palmatine, jatrorrhizin and coptisine. The extraction rate of them is more than 98%. Hydrophobic interaction is one of the main factors, which directly affects the extraction of aqueous two-phase system.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fengwei Wang
AU  - Daowu Wang
AU  - Qingfeng Guo
AU  - Donghui Chen
PY  - 2015/04
DA  - 2015/04
TI  - Study on Distribution Behavior in Aqueous Two-phase System about Four Kinds of Alkaloids in Coptis Chinensis
BT  - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
PB  - Atlantis Press
SP  - 444
EP  - 447
SN  - 2352-5401
UR  - https://doi.org/10.2991/mebe-15.2015.104
DO  - 10.2991/mebe-15.2015.104
ID  - Wang2015/04
ER  -