Electrophysiological Recording of Potassium Currents in Hippocampal Neurons

DOI

10.2991/mebe-15.2015.130How to use a DOI?

Keywords

potassium current; electrophysiology; neuron.

Abstract

Potassium channels are one of the most complex and wide-distributed channels in neurons, which have concentrated more and more attention, because it is an important signal pathway in the neuronal apoptosis and pathogenesis of Alzheimer's disease and neuron apoptosis. In this study whole-cell electrophysiological recording techniques were used to identify potassium currents in cultured rat hippocampal neurons (P7 to P14). The membrane potential was held at 65mV, before the activation of outward potassium currents, the membrane potential was pre-hyperpolarized to 110 mV, and then depolarized to +70 mV in 10 mV steps. We found that a kind of outward delayed potassium current was voltage dependent, and it could be significantly inhibited by 50 mM tetraethyl ammonium (TEA).

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Yuxiang Wu
AU  - Minfang Huang
AU  - Tao Song
AU  - Guodong Xu
PY  - 2015/04
DA  - 2015/04
TI  - Electrophysiological Recording of Potassium Currents in Hippocampal Neurons
BT  - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
PB  - Atlantis Press
SP  - 565
EP  - 568
SN  - 2352-5401
UR  - https://doi.org/10.2991/mebe-15.2015.130
DO  - 10.2991/mebe-15.2015.130
ID  - Wu2015/04
ER  -